Woods Hole Oceanographic Institution

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»Mar Ecol 2000
»Appl Env Microb 2000
»FEMS Microb Ecol 2001
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»Deep-Sea Res 2006
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»Aquat Microb Ecol 2006, 1
»Aquat Microb Ecol 2006, 2
»GCA 2009
»FEMS Microb Ecol 2009
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»GCA 2010
»Org Geochem 2010
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»Env Res Letters 2011
»Org Geochem 2012
»Geomicro accepted

K. Longnecker and E.B. Kujawinski, Using stable isotope probing to characterize differences between free-living and sediment-associated microorganisms in the subsurface, Geomicrobiology Journal, (accepted)

Aquifers are subterranean reservoirs of freshwater with heterotrophic bacterial communities attached to the sediments and free-living in the groundwater. In the present study, mesocosms were used to assess factors controlling the diversity and activity of the subsurface bacterial community. The assimilation of 13C, derived from 13C-acetate, was monitored to determine whether the sediment-associated and free-living bacterial community would respond similarly to the presence of protozoan grazers. We observed a dynamic response in the sediment-associated bacterial community and none in the free-living community. The disparity in these observations highlights the importance of the sediment-associated bacterial community in the subsurface carbon cycle.

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