Woods Hole Oceanographic Institution

Elizabeth B Kujawinski Behn

»Using stable isotope probing to characterize differences between free-living and sediment-associated microorganisms in the subsurface.
»DOM in Lake Superior
»Deepwater Horizon hydrocarbons in the marine environment
»Microbes and marine DOM, Ann. Rev. Mar. Sci. 2011
»Greenland ice sheet outlet glacier: Insights from a new isotope-mixing model
»Groundwater DOM, GCA 2011
»Dispersants & DWH, ES&T 2011
»FT-MS variability in DOM, Org Geochem 2010
»Predatory Flavobacteria, FEMS Microb Ecol 2010
»Greenland Ice Sheet DOM, GCA 2010
»Protozoa and bacteria in aquifers, FEMS Microb Ecol, 2009
»Source markers in DOM, GCA 2009
»Automated data analysis, Anal. Chem. 2006
»Marine DOM and ESI FT-ICR MS; Marine Chem 2004
»DOM extraction by C18; Org. Geochem. 2003
»Black carbon by ESI FT-ICR MS; ES&T 2004
»ESI FT-ICR MS review; Env. Forensics 2002
»Marine protozoan surfactants; Marine Chem. 2002
»ESI MS and NOM; Org. Geochem. 2002
»ESI FT-ICR MS & humic acids; Anal. Chem. 2002
»Protozoan DOM & PCBs; ES&T 2001
»Protozoa & Fe, Th, C; Aquat. Microb. Ecol. 2001
»PCB uptake by protozoa; AEM 2000

E. B. Kujawinski, J. W. Farrington, and J. W. Moffett, Marine protozoa produce organic matter with a high affinity for PCBs during grazing, Environmental Science & Technology 35: 4060-4065, 2001

Processes influencing organic carbon distribution and composition can control the speciation of organic contaminants such as polychlorinated biphenyls (PCBs) and ultimately determine their residence time in aquatic environments.  Protozoan grazers are active in the remineralization and recycling of organic material both in the water column and at the sediment-water interface.  Thus, they influence the quality and quantity of potential PCB binding substrates in the suspended and dissolved phases of aqueous systems.  In this study, common headspace systems were used to compare the chlorobiphenyl-binding affinity of dissolved organic carbon (DOC) in protozoan and bacterial culture filtrates (<0.2 mm) relative to DOC in a seawater control.  Culture filtrates from three marine protozoan species were compared – Uronema sp., Cafeteria sp. and Paraphysomonas imperforata.  Each protozoan species was fed the same bacterial prey, Halomonas halodurans, which was also used as a bacterial control.  Affinities of culture DOC for 14C-3,3’,4,4’-tetrachlorobiphenyl (IUPAC #77) were normalized to DOC and surfactant concentrations.  Values of DOC equilibrium partition coefficients (KDOC) ranged from 104.6 in seawater (Vineyard Sound , MA ) to 105.4 and 105.5 in protist cultures, indicating that grazer-modified DOC was a better sorbent for PCBs than DOC in bacterial or seawater controls.


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