Woods Hole Oceanographic Institution

Elizabeth B Kujawinski Behn

»Using stable isotope probing to characterize differences between free-living and sediment-associated microorganisms in the subsurface.
»DOM in Lake Superior
»Deepwater Horizon hydrocarbons in the marine environment
»Microbes and marine DOM, Ann. Rev. Mar. Sci. 2011
»Greenland ice sheet outlet glacier: Insights from a new isotope-mixing model
»Groundwater DOM, GCA 2011
»Dispersants & DWH, ES&T 2011
»FT-MS variability in DOM, Org Geochem 2010
»Predatory Flavobacteria, FEMS Microb Ecol 2010
»Greenland Ice Sheet DOM, GCA 2010
»Protozoa and bacteria in aquifers, FEMS Microb Ecol, 2009
»Source markers in DOM, GCA 2009
»Automated data analysis, Anal. Chem. 2006
»Marine DOM and ESI FT-ICR MS; Marine Chem 2004
»DOM extraction by C18; Org. Geochem. 2003
»Black carbon by ESI FT-ICR MS; ES&T 2004
»ESI FT-ICR MS review; Env. Forensics 2002
»Marine protozoan surfactants; Marine Chem. 2002
»ESI MS and NOM; Org. Geochem. 2002
»ESI FT-ICR MS & humic acids; Anal. Chem. 2002
»Protozoan DOM & PCBs; ES&T 2001
»Protozoa & Fe, Th, C; Aquat. Microb. Ecol. 2001
»PCB uptake by protozoa; AEM 2000

S. Kim, A. J. Simpson, E. B. Kujawinski, M. A. Freitas and P. G. Hatcher., High resolution electrospray ionization mass spectrometry and 2D solution NMR for the analysis of DOM extracted by C18 solid phase disk, Organic Geochemistry 34: 1325-1335, 2003

We propose and demonstrate an approach involving use of C18 solid phase disk extraction coupled with high resolution mass spectrometry for obtaining non-invasive molecular level information on dissolved organic matter (DOM) from river water. With this approach, DOM extraction from acidified natural water can be achieved rapidly with a simple filtration setup at a remote field site. From total organic carbon and UV-Vis absorbance measurements, we show that a large portion (over 60%) of the original DOM in water is recovered without the interference of salts. NMR analysis indicates that the C18-isolated material has a similar distribution of functional groups as the original DOM but the 2-D NMR details are greatly enhanced. Electrospray ionization mass spectrometry and high resolution mass spectrometry were employed to study DOM at molecular level. Highly resolved mass spectra of DOM (resolving power (delta-m/m50%) > 80,000 at m/z < 600) reveal that there are many pairs of peaks that differ by the exact masses of -H2, -O, or -CH2 indicative of possible homologous series of structures.

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