Woods Hole Oceanographic Institution

Tim Verslycke

»Copepod diapause
»Lobster Shell Disease
»Crustacean molting receptor
»Lobster Shell Disease
»Mysids as test models for endocrine disruption testing
»Chlorotriazines in the Scheldt estuary
»Energy allocation in grasshopper
»Estrogens in Scheldt estuary
»Marsupial development in mysids to evaluate endocrine disruption
»B[a]P effects on steroid metabolism in mysid
»Ciona CYP3 genes
»Methoprene, nonylphenol, and estrone effects on mysid vitellogenesis
»Methoprene effects on mysid molting
»Mysid growth
»Mysid vitellin ELISA
»Mysid vitellin
»An analytical method to detect estrogens in water
»High levels of endocrine disruptors in wild mysid populations
»Energy allocation in wild mysid populations
»Cellular energy allocation validation with scope for growth
»Dolphin delivery prediction
»PhD thesis
»Endocrine disruptor effects on steroid and energy metabolism in mysid
»Mysid review
»TBT effects on steroid metabolism in mysid
»Metal mixture toxicity to mysid
»TBT effects on energy metabolism in mysid
»dichlorobenzene effects in zebrafish
»Ethinylestradiol effects on amphipod sexual development
»Metabolic studies with mysids
»Abiotic stress and energy metabolism in mysid
»Induced vitellogenesis in rainbow trout
»Steroid metabolism in mysid
»Endocrine disruption in freshwater snails
»Invasive mysid in Belgium

Herlinde Noppe, Katia De Wasch, Sofie Poelmans, Nathalie Van Hoof, Tim Verslycke, Colin Janssen, Hubert De Brabander, Development and validation of an analytical method to detect estrogens in water, Analytical and Bioanalytical Chemistry 382:91-98, 2005

An analytical procedure enabling routineanalysis of four environmental estrogens at concentrationsbelow 1 ng/L in estuarine water samples has been developed and validated. The method includes extraction of water samples using solid-phase extraction discs and detection by gas chromatography (GC) with tandem mass spectrometry (MS–MS) inelectron-impact (EI) mode. The targeted estrogens included 17α- and 17ß -estradiol (αE2, ßE2), estrone (E1), and 17α -ethinylestradiol (EE2), all known environmentalendocrine disruptors. Method performance characteristics, for example trueness, recovery, calibration, precision, accuracy, limit of  quantification (LOQ), and the stability of the compounds are presented for each of the selected estrogens. Application of the procedure to water samples from the Scheldtestuary (Belgium – The Netherlands), a polluted estuary with reported incidences of environmental endocrine disruption, revealed that E1 was detected mostfrequently at concentrations up to 7 ng/L. αE2 was detected once only and concentrations of ßE2 and EE2 were below the LOQ.

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