Woods Hole Oceanographic Institution

Tim Verslycke

»Copepod diapause
»Lobster Shell Disease
»Crustacean molting receptor
»Lobster Shell Disease
»Mysids as test models for endocrine disruption testing
»Chlorotriazines in the Scheldt estuary
»Energy allocation in grasshopper
»Estrogens in Scheldt estuary
»Marsupial development in mysids to evaluate endocrine disruption
»B[a]P effects on steroid metabolism in mysid
»Ciona CYP3 genes
»Methoprene, nonylphenol, and estrone effects on mysid vitellogenesis
»Methoprene effects on mysid molting
»Mysid growth
»Mysid vitellin ELISA
»Mysid vitellin
»An analytical method to detect estrogens in water
»High levels of endocrine disruptors in wild mysid populations
»Energy allocation in wild mysid populations
»Cellular energy allocation validation with scope for growth
»Dolphin delivery prediction
»PhD thesis
»Endocrine disruptor effects on steroid and energy metabolism in mysid
»Mysid review
»TBT effects on steroid metabolism in mysid
»Metal mixture toxicity to mysid
»TBT effects on energy metabolism in mysid
»dichlorobenzene effects in zebrafish
»Ethinylestradiol effects on amphipod sexual development
»Metabolic studies with mysids
»Abiotic stress and energy metabolism in mysid
»Induced vitellogenesis in rainbow trout
»Steroid metabolism in mysid
»Endocrine disruption in freshwater snails
»Invasive mysid in Belgium

An Ghekiere, Tim Verslycke, Colin Janssen, Effects of methoprene, nonylphenol, and estrone on the vitellogenesis of the mysid Neomysis integer, General and Comparative Endocrinology 147(2): 190-195, 2006

The induction of the female-specific protein, vitellogenin, in male fish is a well-established endpoint to assess exposure to estrogen-like chemicals. The use of vitellogenesis as a biomarker for xenobiotic exposure in egg-laying invertebrates, however, is still relatively unexplored. Recently, we developed a quantitative enzyme-linked immunosorbent assay (ELISA) for vitellin in Neomysis integer (Crustacea: Mysidacea) to study mysid vitellogenesis and its potential disruption by xenobiotics. In this study, gravid mysids were exposed to methoprene, nonylphenol, and estrone for 96 h. All methoprene-exposed (0.01, 1, and 100 μg/L) animals had lower vitellin levels compared to the control animals, though this effect was not statistically significant. Exposure to nonylphenol resulted in significantly induced vitellin levels in the lowest exposure concentration (0.01 μg/L), whereas no effects were observed at higher concentrations. Estrone significantly decreased vitellin levels at the highest test concentration (1 μg/L). These results indicate that mysid vitellogenesis can be disrupted following chemical exposure. Difficulties in the interpretation of the observed chemical-specific and concentration-specific responses in this study highlight the need for a better understanding of hormone regulation of crustacean vitellogenesis.

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