Woods Hole Oceanographic Institution

»Bioavailability of soil organic matter and microbial community dynamics upon permafrost thaw
»7000 years of virus-host molecular dynamics in the Black Sea
»Preservation potential of ancient DNA in Pleistocene marine sediments: Implications for paleoenvironmental reconstructions
»Source-specific variability in post-depositional DNA preservation with potential implications for DNA-based paleecological records
»Exploring preserved ancient dinoflagellalte and haptophyte DNA signatures to infer ecological and environmental conditions during sapropel S1 formation in the eastern Mediterranean
»Ancient DNA in lake sediment records
»Vertical distribution of metabolically active eukaryotes in the water column and sediments of the Black Sea
»DNA and lipid molecular stratigraphic records of haptophyte succession in the Black Sea during the Holocene
»Diversity of Archaea and potential for crenarchaeotal nitrification of group 1.1a in the rivers Rhine and TĂȘt
»Holocene sources of fossil BHPs
»An unusual 17[α],21[β](H)-bacteriohopanetetrol in Holocene sediments from Ace Lake (Antarctica)
»Holocene sources of organic matter in Antarctic fjord
»Variations in spatial and temporal distribution of Archaea in the North Sea
»Archaeal nitrifiers in the Black Sea
»Pleistocene Mediterranean sapropel DNA
»Rapid sulfurisation of highly branched isoprenoid (HBI) alkenes in sulfidic Holocene sediments
»Aerobic and anaerobic methanotrophs in the Black Sea water column
»Fossil DNA in Cretaceous Black Shales: Myth or Reality?
»Sulfur and methane cycling during the Holocene in Ace Lake (Antarctica)
»Ancient algal DNA in the Black Sea
»Archaeal nitrification in the ocean
»Characterization of microbial communities found in the human vagina by analysis of terminal restriction fragment length polymorphisms of 16S rRNA genes
»Biomarker and 16S rDNA evidence for anaerobic oxidation of methane and related carbonate precipitation in deep-sea mud volcanoes of the Sorokin Trough, Black Sea
»Temperature-dependent variation in the distribution of tetraether membrane lipids of marine Crenarchaeota: Implications for TEX86 paleothermometry
»Paleoecology of algae in Ace Lake
»Evolution of the methane cycle in Ace Lake (Antarctica) during the Holocene: Response of methanogens and methanotrophs to environmental change
»Ongoing modification of Mediterranean Pleistocene sapropels mediated by prokaryotes.
»Microbial communities in the chemocline of a hypersaline deep-sea basin (Urania basin, Mediterranean Sea)
»Functional exoenzymes as indicators of metabolically active bacteria in 124,000-year-old sapropel layers of the Eastern Mediterranean Sea
»Specific detection of different phylogenetic groups of chemocline bacteria based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments
»Analysis of subfossil molecular remains of purple sulfur bacteria in a lake sediment
»Effects of nitrate availability and the presence of Glyceria maxima the composition and activity of the dissimilatory nitrate-reducing bacterial community
»Microbial activities and populations in upper sediment and sapropel layers

Stadnitskaia, A., G. Muyzer, B. Abbas, M. J. L. Coolen, E. C. Hopmans, M. Baas, T. C. E. Van Weering, M. K. Ivanov, E. Poludetkina and J. S. Sinninghe Damsté, Biomarker and 16S rDNA evidence for anaerobic oxidation of methane and related carbonate precipitation in deep-sea mud volcanoes of the Sorokin Trough, Black Sea, Mar. Geol., 217(1-2), 67-96, 2005

Many mud volcanoes were recently discovered in the euxinic bottom waters of the Sorokin Trough (NE Black Sea). Three of them, i.e., NIOZ, Odessa, and Kazakov, were selected for a detailed biogeochemical investigation. Four methane-related carbonate crusts covered with microbial mats, and sediments (‘mud breccia’) from these mud volcanoes were collected during the 11th Training-Through-Research cruise (TTR-11) in 2001, the first finding of methanotrophic microbial mats associated with authigenic carbonates in the deep Black Sea. We measured the concentrations and δ13C values of methane and specific archaeal and bacterial lipids, and determined archaeal and bacterial 16S rRNA gene sequences. The δ13C of the microbial lipids reflected the carbon isotopic values of the methane, indicating that methane was the main carbon source for microorganisms inducing carbonate formation. Anaerobic oxidation of methane (AOM) in these settings was performed by archaea affiliated with the so-called ANME-1 group. None of the identified archaeal sequences were closely related to known methanogens. The combined 16S rRNA gene sequence and biomarker data revealed a distinct difference in archaeal assemblage between the carbonate crusts and mud breccias. Besides gene sequences of sulfate-reducing bacteria, DNA analysis of bacterial communities revealed a diversity of bacteria with apparent contrasting metabolic properties. The methane utilization via AOM processes was detected in the uppermost sediments where it subsequently induces authigenic carbonate precipitation most probably below seafloor. The results of integrated biomarker and 16S rRNA gene study reveal a crucial role of AOM processes in formation of authigenic carbonates in methane seep environments. Full text of article can be viewed here.

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