Woods Hole Oceanographic Institution

Elizabeth B Kujawinski Behn

»Using stable isotope probing to characterize differences between free-living and sediment-associated microorganisms in the subsurface.
»DOM in Lake Superior
»Deepwater Horizon hydrocarbons in the marine environment
»Microbes and marine DOM, Ann. Rev. Mar. Sci. 2011
»Greenland ice sheet outlet glacier: Insights from a new isotope-mixing model
»Groundwater DOM, GCA 2011
»Dispersants & DWH, ES&T 2011
»FT-MS variability in DOM, Org Geochem 2010
»Predatory Flavobacteria, FEMS Microb Ecol 2010
»Greenland Ice Sheet DOM, GCA 2010
»Protozoa and bacteria in aquifers, FEMS Microb Ecol, 2009
»Source markers in DOM, GCA 2009
»Automated data analysis, Anal. Chem. 2006
»Marine DOM and ESI FT-ICR MS; Marine Chem 2004
»DOM extraction by C18; Org. Geochem. 2003
»Black carbon by ESI FT-ICR MS; ES&T 2004
»ESI FT-ICR MS review; Env. Forensics 2002
»Marine protozoan surfactants; Marine Chem. 2002
»ESI MS and NOM; Org. Geochem. 2002
»ESI FT-ICR MS & humic acids; Anal. Chem. 2002
»Protozoan DOM & PCBs; ES&T 2001
»Protozoa & Fe, Th, C; Aquat. Microb. Ecol. 2001
»PCB uptake by protozoa; AEM 2000

Minor, E. C., C. J. Steinbring, K. Longnecker+, and E. B. Kujawinski., Characterization of dissolved organic matter in Lake Superior and its watershed using ultrahigh resolution mass spectrometry., Organic Geochemistry 43: 1-11, 2012

With the advent of ultrahigh resolution mass spectrometry (MS), recent studies have begun to resolve molecular relationships between terrestrial and aquatic dissolved organic matter (DOM) in rivers, estuaries, mangrove swamps and their receiving oceans and lakes. Here, we extend ultrahigh resolution MS techniques to Lake Superior, the largest freshwater lake in the world by area. Solid-phase extracted samples from the western arm of the lake and its watershed, including swamp, creek, river, lake–river confluence and offshore lake sites were compared using electrospray ionization (ESI), Fourier transform ion cyclotron resonance MS (FT-ICR-MS). Results were analyzed using cluster analysis and van Krevelen diagrams. Chemical similarity appears related to hydrological proximity, terrestrial impact and flow conditions. For example, higher and lower flow samples from the same stream differ from one another. Toivola Swamp, Lake Superior and the south shore river have diverse arrays of unique molecular formulae relative to the north shore river and stream sampled in the data set. The lake’s unique elemental formulae, relative to its watershed samples, are primarily in the lignin-like and reduced hydrocarbon regions of van Krevelen diagrams. The ESI-amenable lake DOM also has a higher proportion of formulae containing nitrogen or sulfur relative to the other samples. The degree of overlap among formulae within the data set is consistent with previous ESI FT-ICR-MS characterization of terrestrial, estuarine and marine OM. There appears to be a conserved portion of formulae across natural OM samples, perhaps because these compounds are intrinsically refractory or because they are commonly generated as products of natural reworking processes. Full text published in Organic Geochemistry can be found Here

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