Woods Hole Oceanographic Institution

Tim Verslycke

»Copepod diapause
»Lobster Shell Disease
»Crustacean molting receptor
»Lobster Shell Disease
»Mysids as test models for endocrine disruption testing
»Chlorotriazines in the Scheldt estuary
»Energy allocation in grasshopper
»Estrogens in Scheldt estuary
»Marsupial development in mysids to evaluate endocrine disruption
»B[a]P effects on steroid metabolism in mysid
»Ciona CYP3 genes
»Methoprene, nonylphenol, and estrone effects on mysid vitellogenesis
»Methoprene effects on mysid molting
»Mysid growth
»Mysid vitellin ELISA
»Mysid vitellin
»An analytical method to detect estrogens in water
»High levels of endocrine disruptors in wild mysid populations
»Energy allocation in wild mysid populations
»Cellular energy allocation validation with scope for growth
»Dolphin delivery prediction
»PhD thesis
»Endocrine disruptor effects on steroid and energy metabolism in mysid
»Mysid review
»TBT effects on steroid metabolism in mysid
»Metal mixture toxicity to mysid
»TBT effects on energy metabolism in mysid
»dichlorobenzene effects in zebrafish
»Ethinylestradiol effects on amphipod sexual development
»Metabolic studies with mysids
»Abiotic stress and energy metabolism in mysid
»Induced vitellogenesis in rainbow trout
»Steroid metabolism in mysid
»Endocrine disruption in freshwater snails
»Invasive mysid in Belgium

Tim Verslycke, Gert F. Vandenbergh, Bram Versonnen, Katrien Arijs, Colin R. Janssen, Induction of vitellogenesis in 17a-ethinylestradiol-exposed rainbow trout (Oncorhynchus mykiss): a method comparison, Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 132(4): 483-492, 2002

Juvenile rainbow trout, Oncorhynchus mykiss, were exposed to the synthetic estrogen 17a-ethinylestradiol (EE2) through injection (1, 10, 25 and 50 µg EE2/g fish/week) and via water exposure (1, 10 and 100 ng EE2/l). After seven (injection and water exposure) and 14 days (only for water exposure), blood and plasma vitellogenin concentrations were quantified using indirect endpoints, i.e. plasma alkaline-labile phosphorus (ALP), plasma protein and plasma calcium. In addition, the relative gonad (GSI) and liver weight (HSI) were recorded. Actual plasma vitellogenin concentrations were measured with an enzyme immunoassay. Only fish injected with 50 µg EE2/g fish had a significantly higher gonad weight. No concentration-dependent changes in the HSI were detected in fish exposed via the water, but a significant dose-dependent increase of the HSI was observed in fish injected with EE2. Exposure of rainbow trout to EE2 had a significant effect on all tested plasma parameters. Plasma protein, phosphoprotein and calcium concentrations were significantly higher after two weeks exposure to 100 ng EE2/l. Fish injected with 10, 25 and 50 µg EE2/g fish exhibited increased plasma protein concentrations after 1 week. Compared to the controls, plasma ALP and calcium levels were significantly higher in all injected fish. A significant and positive correlation was observed between all three plasma parameters and between these indirect parameters and the actual plasma vitellogenin concentrations. These findings indicate that both the plasma ALP and the plasma calcium assay have a similar sensitivity as that of available antibody-based assays (EIA), at least in EE2 exposure studies, and thus these assays can provide a rapid, simple and cost-effective alternative to available immunoassays. doi:10.1016/S1532-0456(02)00111-4

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