Woods Hole Oceanographic Institution

Tim Verslycke

»Copepod diapause
»Lobster Shell Disease
»Crustacean molting receptor
»Lobster Shell Disease
»Mysids as test models for endocrine disruption testing
»Chlorotriazines in the Scheldt estuary
»Energy allocation in grasshopper
»Estrogens in Scheldt estuary
»Marsupial development in mysids to evaluate endocrine disruption
»B[a]P effects on steroid metabolism in mysid
»Ciona CYP3 genes
»Methoprene, nonylphenol, and estrone effects on mysid vitellogenesis
»Methoprene effects on mysid molting
»Mysid growth
»Mysid vitellin ELISA
»Mysid vitellin
»An analytical method to detect estrogens in water
»High levels of endocrine disruptors in wild mysid populations
»Energy allocation in wild mysid populations
»Cellular energy allocation validation with scope for growth
»Dolphin delivery prediction
»PhD thesis
»Endocrine disruptor effects on steroid and energy metabolism in mysid
»Mysid review
»TBT effects on steroid metabolism in mysid
»Metal mixture toxicity to mysid
»TBT effects on energy metabolism in mysid
»dichlorobenzene effects in zebrafish
»Ethinylestradiol effects on amphipod sexual development
»Metabolic studies with mysids
»Abiotic stress and energy metabolism in mysid
»Induced vitellogenesis in rainbow trout
»Steroid metabolism in mysid
»Endocrine disruption in freshwater snails
»Invasive mysid in Belgium

Tim Verslycke, Sofie Poelmans, Katia De Wasch, Hubert F. De Brabander, Colin R. Janssen, Testosterone and energy metabolism in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) following exposure to endocrine disruptors, Environmental Toxicology and Chemistry 23 (5): 1289-1296 , 2004

A diverse set of reference compounds suspected of having an endocrine-disrupting mode of action were tested for acute toxicity to the estuarine mysid Neomysis integer (Crustacea:Mysidacea), i.e. testosterone, flutamide, ethinylestradiol, precocene, nonylphenol, fenoxycarb and methoprene. N. integer was very sensitive to all tested compounds, with 96-h median lethal concentrations in a narrow range between 0.32 and 1.95 mg/L. The pesticides methoprene and fenoxycarb, both synthetic insect juvenile hormone analogs, were most toxic to N. integer. In addition, the short-term sublethal effects of methoprene and nonylphenol (an estrogen agonist) on the energy and steroid metabolism of N. integer were evaluated. Both compounds significantly affected energy and testosterone metabolism of N. integer at concentrations below acute toxicity levels. Energy consumption in methoprene and nonylphenol-exposed mysids was significantly induced at 100 µg/L, resulting in a lower cellular energy allocation (CEA) in these animals. Testosterone phase I metabolism was affected at 10 µg/L, whereas glycosylation was the most important phase II pathway affected in mysids exposed to 100 µg/L of both compounds. Methoprene exposure resulted in a concentration-dependent increase in the metabolic androgenization ratio (MAR). Mysids exposed to 10 g/L nonylphenol had a significantly higher MAR. The present study indicates that energy and testosterone metabolism of mysids, as endpoints, are able to detect endocrine disruptive activity of chemicals following short-term exposure to environmentally realistic levels of endocrine disruptors.

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