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Woods Hole Oceanographic Institution

Marco Coolen

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Publications
»Bioavailability of soil organic matter and microbial community dynamics upon permafrost thaw
»7000 years of virus-host molecular dynamics in the Black Sea
»Preservation potential of ancient DNA in Pleistocene marine sediments: Implications for paleoenvironmental reconstructions
»Source-specific variability in post-depositional DNA preservation with potential implications for DNA-based paleecological records
»Exploring preserved ancient dinoflagellalte and haptophyte DNA signatures to infer ecological and environmental conditions during sapropel S1 formation in the eastern Mediterranean
»Ancient DNA in lake sediment records
»Vertical distribution of metabolically active eukaryotes in the water column and sediments of the Black Sea
»DNA and lipid molecular stratigraphic records of haptophyte succession in the Black Sea during the Holocene
»Diversity of Archaea and potential for crenarchaeotal nitrification of group 1.1a in the rivers Rhine and TĂȘt
»Holocene sources of fossil BHPs
»An unusual 17[α],21[β](H)-bacteriohopanetetrol in Holocene sediments from Ace Lake (Antarctica)
»Holocene sources of organic matter in Antarctic fjord
»Variations in spatial and temporal distribution of Archaea in the North Sea
»Archaeal nitrifiers in the Black Sea
»Pleistocene Mediterranean sapropel DNA
»Rapid sulfurisation of highly branched isoprenoid (HBI) alkenes in sulfidic Holocene sediments
»Aerobic and anaerobic methanotrophs in the Black Sea water column
»Fossil DNA in Cretaceous Black Shales: Myth or Reality?
»Sulfur and methane cycling during the Holocene in Ace Lake (Antarctica)
»Ancient algal DNA in the Black Sea
»Archaeal nitrification in the ocean
»Characterization of microbial communities found in the human vagina by analysis of terminal restriction fragment length polymorphisms of 16S rRNA genes
»Biomarker and 16S rDNA evidence for anaerobic oxidation of methane and related carbonate precipitation in deep-sea mud volcanoes of the Sorokin Trough, Black Sea
»Temperature-dependent variation in the distribution of tetraether membrane lipids of marine Crenarchaeota: Implications for TEX86 paleothermometry
»Paleoecology of algae in Ace Lake
»Evolution of the methane cycle in Ace Lake (Antarctica) during the Holocene: Response of methanogens and methanotrophs to environmental change
»Ongoing modification of Mediterranean Pleistocene sapropels mediated by prokaryotes.
»Microbial communities in the chemocline of a hypersaline deep-sea basin (Urania basin, Mediterranean Sea)
»Functional exoenzymes as indicators of metabolically active bacteria in 124,000-year-old sapropel layers of the Eastern Mediterranean Sea
»Specific detection of different phylogenetic groups of chemocline bacteria based on PCR and denaturing gradient gel electrophoresis of 16S rRNA gene fragments
»Analysis of subfossil molecular remains of purple sulfur bacteria in a lake sediment
»Effects of nitrate availability and the presence of Glyceria maxima the composition and activity of the dissimilatory nitrate-reducing bacterial community
»Microbial activities and populations in upper sediment and sapropel layers


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Herfort, L., Schouten, S., Abbas, B., Veldhuis, M.J.W., Coolen, M.J.L., Wuchter, C. et al., Variations in spatial and temporal distribution of Archaea in the North Sea in relation to environmental variables, FEMS Microbiol Ecol 62: 242-257, 2007

The spatial and temporal distribution of pelagic Archaea was studied in the southern North Sea by rRNA hybridization, sequencing and quantification of 16S rRNA gene and membrane lipid analyses and related to physical, chemical and biological parameters to determine the factors influencing archaeal biogeography. A clear temporal variability was observed, with marine Crenarchaeota (Group I.1a) being relatively more abundant in winter and Euryarchaeota dominating the archaeal assemblage in spring and summer. Spatial differences in the lateral distribution of Crenarchaeota were also evident. In fact, their abundance was positively correlated with the copy number of the gene encoding the alpha subunit of crenarchaeotal ammonia monooxygenase (amoA) and with concentrations of ammonia, nitrate, nitrite and phosphorus. This suggests that most Crenarchaeota in the North Sea are nitrifiers and that their distribution is determined by nutrient concentrations. However, Crenarchaeota were not abundant when larger phytoplankton (>3 microm) dominated the algal population. It is hypothesized that together with nutrient concentration, phytoplankton biomass and community structure can predict crenarchaeotal abundance in the southern North Sea. Euryarchaeotal abundance was positively correlated with chlorophyll a concentrations, but not with phytoplankton community structure. Whether this is related to the potential of Euryarchaeota to perform aerobic anoxygenic phototrophy remains to be shown, but the conspicuous seasonal distribution pattern of Crenarchaeota and Euryarchaeota suggests that they occupy a different ecological niche. Full text of article can be found here.

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