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Sample preparation task 10, by E. Sichel
on July 29, 2002
PSI-326, Stoelting bone cement #51458, 3M
Fastcure 051135-08107
Assistance provided by Ann Bodio, student technician.
Enid and Ann working on a very hot day
Three dozen live scallops were scrubbed free
of fouling, blotted dry with a paper towel, and placed on
a dry bench top. A drop of anhydrous ethyl alcohol was placed
on the top of each shell. Then the adhesive was daubed on
the dry spot on the shell. Then the netting was pressed into
the adhesive. The adhesive was allowed to dry for 15 minutes
in air on the bench top. Then the animals were returned to
the seawater tank. The temperature of the seawater in the
tank today was 23 degrees C.
This preparation differs from previous ones
because
- The netting was on top of the scallop instead
of underneath it for the 15 minute cure time. Although the
scallop spits, claps, and drools, the adhesive bond was
not wetted by the animals's antics.
- The shell was dried by the evaporation of
a drop of ethyl alcohol. The alcohol was anhydrous and poured
into a dispensing bottle a half hour before its use, but
the air is so humid that the alcohol picks up water even
while on the bench top in a squeeze bottle.
- Two of the adhesives were applied to shells
held closed with clothes pins while the adhesive set up.
(Stoelting bone cement and 3M Fastcure 051135-08107)
Follow-up report after one week
The bonds were in good condition, but a lot
of the animals had died. We attribute the loss to use of the
ethyl alcohol to dry the shells. We believe that the alcohol
flowed across the shell and contacted the soft tissue.
| adhesive |
after 1 week |
| PSI-326 |
9 out of 12 attached (4 empty) |
| Stoelting bone cement |
11 out of 12 attached (4 empty) |
| 3M Fastcure 051135-08107 |
12 out of 12 attached (7 empty) |
 |
 |
 |
| PSI-326 |
Stoelting cement |
3M Fastcure |
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