Accuri C6 consumables, manuals, etc.

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Related Files

» C6 manual

» BC aquatic uses whitepaper

» CFlowPlus_1.0.227.4.zip
Zip file with the C6 operating/analysis software (from CD, 76MB)

» MSDS KR200 Sodium Hypochlorite

» MSDS KR220 Bacteriostat

» MSDS KR225 Cleaning Solution

» MSDS KR235 ExtendingFlowCellCleaningSolution

Accuri C6 consumables



Consumables (fluids): (MSDS linked at the right -->)

  1. Cleaning solution, KR-225 (citric acid).  To fill the cleaning solution bottle (green line), add 3 ml of 10-30% citric acid and fill with DI. There are two options here: Buy from Accuri, or make your own citric acid solution. It is cheaper and easier to make your own. Prepare before the cruise by adding pre-weighed citric acid to an empty polycarbonate bottle, then filling with DI when you are on the ship (make the ratios work so that you get 10-30% of this stock solution; it will last the entire cruise). You will fill the bottle 1 to 2 times/week when using the Accuri regularly.
  2. Decontamination solution, KR-200 (NaOCl, sodium hypochlorite, bleach). The decontamination bottle (yellow line) is filled about 1 time/week. If using the BD bottles (expensive), add 20 ml of 5% sodium hypochlorite (one full BD/Accuri vial is about 5% NaOCl) to the Accuri’s decontamination bottle. Fill the Accuri’s decon bottle up with DI water, which will give you ~0.5% NaOCl final strength. This decontamination solution is essentially just bleach, so you can buy high-grade NaOCl instead and mix it to the appropriate final solution. Do not use commercial bleaches (Clorox) as these have perfumes and whiteners that can affect the measurements. Although we are not running bio-hazardous material, we should always run with full strength decontamination solution (my opinion). The cleaning solution alone does nothing to keep muck from growing inside the accuri.
  3. Sheath fluid. To fill sheath fluid bottle (blue line), add one bottle of KR-220, bacteriostatic solution concentrate (a cocktail of NaOH, NaF, EDTA), and fill with DI water. This happens less than 1/week.
  4. Waste container. Empty waste container (red line) when full! Down the drain or into waste collection, per rules of whichever ship you’re on.
  5. Extended flow cell cleaning solution (KR-235, 1-5% ethyl alcohol). Can be bought from BD.


Consumables (parts):


  1. Peristaltic pump tubes.  There are two pumps inside the housing, up behind the laser. These pump tubes need to be replaced periodically.
  2. Filters inside bottles. Replace intermittently.
  3. Sheath fluid filter. Sits underneath the spare parts bin inside the instrument. Perhaps wise to replace it at the beginning of each cruise.




Beads

Multi-Peak beads: for thorough check-ups

Spherotech 6-peak Validation beads: FL4 3 microns

Catalog 653145 link  about $300

Secondary beads: for daily check-ups

2.5 micron green beads no longer available: PeakFlow TM Green flow cytometry reference beads, 2.5 µm, 515 nm emission. P-14827. Lot 63B1-3. Link

Best substitute is 6 micron beads (originally Molecular Probes P-14828, now ThermoFisher C16508) link: http://www.thermofisher.com/order/catalog/product/C16508

Use BD/Accuri Multi-peak beads for more detailed assessments (see manual). E.g., Spherotech 6-peak validation beads, link.



Consumables: sampling

2 ml sampling vials: Fisher 02-681-258

 

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Last updated July 22, 2016
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