Fredricks / Van Mooy lab data files
Oxylipin / lipid analysis
General information - all analyses
Total lipid extract obtained following a modified Bligh & Dyer protocol. BHT (butylated hydroxyl toluene) added as an antioxidant, DNP-PE added as an internal std (dinitrophenylphosphatidylethanolamine, Avanti polar lipids).
Analyzed on Thermo Exactive plus Orbitrap mass spectrometer (electrospray ionization) coupled to an Agilent 1200 HPLC. Chromatography follows Hummel et al. (reverse phase, C8 column).
Raw data is presented as *.raw files from Xcalibur 2.2 software (Thermo). If Xcalibur is not available, conversion utilities are available on the web which may convert raw files to a more accessible format.
Excel files are worked-up output from either Quanbrowser (Thermo) or Sieve. More information is given in these files.
Analyzed “big 9” lipids on Thermo TSQ Vantage triple quadrupole mass spectrometer. Electrospray ionization, chromatography – normal phase on a diol column as published in Lipids:
1. Oxylipin analysis - Diatom cultures exposed to H2O2
Samples from: Kim Thamatrakoln / Kay Bidle (Rutgers), extracted and analyzed by Helen Fredricks, Sieve data processing Helen Fredricks and Bethanie Edwards (mostly BE!)
5 cultures: T pseudonana, C socialis, C tenuissimus 2-10, C tenuissimus 2-6, C lorenzianus: Each exposed to 0, 30, 150 μM H2O2Sample List (Excel file)
Data Work-ups (Excel file)
ftp link to raw data files: ftp://ftp.whoi.edu/pub/science/MCG/gbmf/VanMooy/OxylipinAnalysis