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Oxylipin and Lipid analysis

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Fredricks / Van Mooy lab data files

Oxylipin / lipid analysis

General information  - all analyses

Total lipid extract obtained following a modified Bligh & Dyer protocol. BHT (butylated hydroxyl toluene) added as an antioxidant, DNP-PE added as an internal std (dinitrophenylphosphatidylethanolamine, Avanti polar lipids).

Oxylipin analysis

Analyzed on Thermo Exactive plus Orbitrap mass spectrometer (electrospray ionization) coupled to an Agilent 1200 HPLC. Chromatography follows Hummel et al. (reverse phase, C8 column).

Raw data is presented as *.raw files from Xcalibur 2.2 software (Thermo). If Xcalibur is not available, conversion utilities are available on the web which may convert raw files to a more accessible format.

Excel files are worked-up output from either Quanbrowser (Thermo) or Sieve. More information is given in these files.

Lipid analysis

Analyzed “big 9” lipids on Thermo TSQ Vantage triple quadrupole mass spectrometer. Electrospray ionization, chromatography – normal phase on a diol column as published in Lipids:

*Popendorf, K.J., *Fredricks, H.F., and Van Mooy, B.A.S.  (2013) Molecular-ion independent quantitation of intact polar diacylglycerolipids in marine plankton using triple quadrupole MS. Lipids 48: 185-195; doi:10.1007/s11745-012-3748-0.

 

1. Oxylipin analysis - Diatom cultures exposed to H2O2

Samples from: Kim Thamatrakoln / Kay Bidle (Rutgers), extracted and analyzed by Helen Fredricks, Sieve data processing Helen Fredricks and Bethanie Edwards (mostly BE!)

5 cultures: T pseudonana, C socialis, C tenuissimus 2-10, C tenuissimus 2-6, C lorenzianus: Each exposed to 0, 30, 150 μM H2O2

Sample List (Excel file)
Data Work-ups (Excel file)
ftp link to raw data files: ftp://ftp.whoi.edu/pub/science/MCG/gbmf/VanMooy/OxylipinAnalysis

Last updated: April 24, 2014
 


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