Woods Hole Oceanographic Institution

Annette M. Hynes
Annette M. Hynes's photoAnnette M. Hynes

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Research Interests
I am studying diversity of the nitrogen-fixing cyanobacterium Trichodesmium and its potential for niche differentiation. I am characterizing strains in the Woods Hole culture collection with Dr. John Waterbury using microscopy, absorption spectra of phycobiliproteins, and phylogenetic analysis of sequences from three genetic markers.  I used my sequences to develop a quantitative polymerase chain reaction (qPCR) protocol to identify and enumerate different clades of Trichodesmium from field samples obtained from the Equatorial Atlantic and the western Pacific Oceans.  With Dr. Scott Doney I am working on a stochastic competition model to investigate the factors influencing the diversity of N2 fixers.

  • Computer Programs and Languages
    • MATLAB
    • UNIX
    • LaTEX
  • Microbiological Techniques
    • Microscopy (phase contrast, DIC, epifluorescence)
    • Flow cytometry
    • Culturing diazotrophic cyanobacteria Trichodesmium and Crocosphaera
    • Acetylene reduction measurement of N2 fixation
  • Molecular Techniques
    • Polymerase chain reaction (PCR)
    • Real-time PCR
    • Sequencing
    • Enzyme-labeled fluorescence (ELF)

  • B.S. Biological Sciences and Mathematics, University of Nebraska-Lincoln, Lincoln, NE, 1998
  • Ph.D. Biological Oceanography, Massachusetts Institute of Technology-Woods Hole Oceanographic Institution Joint Program, Woods Hole, MA, 2009

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