Woods Hole Oceanographic Institution
Cruise Planning Synopsis
AT26-23 | |
Ship | |
| R/V Atlantis | |
Vehicles | |
| HOV Alvin | |
Cruise Party | |
| Peter Girguis: Principal Investigator 16 Divinity Ave room 3085 Cambridge, MA United States 02138 +1 617 496 8328 pgirguis@oeb.harvard.edu Jeremy Rich: Principal Investigator Brown University Providence, RI USA 02912 +1 401 863 6748 jeremy_rich@brown.edu Kathleen Scott: Principal Investigator University of South Florida 4202 East Fowler Avenue Tampa, FL USA 33620 +1 813 480 3908 kmscott@usf.edu Stefan Sievert: Chief Scientist, Principal Investigator Woods Hole Oceanographic Institution Watson Building 207, MS#52 Woods Hole, Ma. USA 02543 +1 508 289 2305 ssievert@whoi.edu Craig Taylor: Principal Investigator Woods Hole Oceanographic Institution Watson Building 209, MS#52 Woods Hole, Ma. USA 02543 +1 508 289 2354 ctaylor@whoi.edu Scott White: Principal Investigator University of South Carolina USA +1 803 777 6304 swhite@geol.sc.edu |
Departure: Nov 2, 2014 | |
| Manzanillo Mexico | |
Arrival: Nov 26, 2014 | |
| Puntarenas Costa Rica | |
Operations Area | |
| 9ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂð North Integrated Study Site | |
| Lat/Lon: 9° 50.0′ N / 104° 18.0′ W | |
| Depth Range: 2500 / 2600 | |
| Will the vessel be operating within 200 NM of a foreign country? | no |
Science Objectives | |
Sievert/Taylor/Rich: Knowledge of the in situ metabolism of microorganisms carrying out CO2-fixation at deep-sea hydrothermal vents is very limited. Particularly lacking are studies measuring rates of autotrophic carbon fixation in situ, which is a measurement ultimately needed to constrain production in these ecosystems. Although recent data suggests that nitrate reduction either to N2 (denitrification) or to NH4+ (dissimilatory reduction of nitrate to ammonium, DNRA) might be responsible for a significant fraction of chemoautotrophic production, NO3--reduction rates have never been measured in situ at hydrothermal vents. We hypothesize that chemoautrophic growth is strongly coupled to nitrate respiration in vent microbial communities. As part of this cruise, we are going to deploy and test a newly developed robotic micro-laboratory, the Vent-Time Series Submersible Incubation Device (Vent-TSSID) for measuring rates of relevant metabolic processes at hydrothermal vents at both in situ pressures and temperatures. Scott and Girguis: The relative importance of the two carbon fixation pathways (CBB, rTCA) in Riftia is impossible to infer from the existing data. Bulk stable carbon isotopic compositions, and the presence and activities of enzymes allied to rTCA and CBB in Riftia symbionts, do not definitively answer whether one or both pathways are operational in a single individual, and the contribution of each pathway to net carbon fixation. The objective of this study is to clarify whether the activity of these pathways in Riftia are regulated by environmental conditions (e.g., the relative abundance of redox substrates), internal heterogeneity, or whether both pathways operate simultaneously. | |
Science Activities | |
Sievert/Taylor/Rich: Our component of the cruise has 12 dives with DSV Alvin, to allow time for deployment and collection of experiments and for sampling of discrete vents along the axis of the 9°46’N to 9°53’N segment of the East Pacific Rise (EPR). We will focus our activities at Crab Spa, a diffuse flow vent site near Tica, and deploy experiments and sampling equipment, including the Vent-TSSID and a large volume pump (LVP). During the course of the cruise we will perform several deployments of the Vent-TSSID, as well as collect biomass from fluids and biofilms (deployment/recover experimental microbial colonizers) by utilizing a the LVP. Both the Vent-TSSID and the LVP will be deployed as an elevator. It is planned to deploy the instruments and bring them close to the deployment site the night before a dive, and then to position the instruments at the site with Alvin the next day. Alvin will be releasing the instrument either on the same dive or any subsequent dive, after which the instrument will be picked up at the surface. Furthermore, we will carry out additional opportunistic sampling of other diffuse-flow vents. Fluid samples for chemical analyses will be collected at each deployment site using isobaric gas-tight samplers, which will also be used to sample fluids from the focus site for the shipboard incubations. Finally, we will deploy/recover experimental microbial colonizers to collect microbial biofilms, as well as collect Riftia for subsequent ‘omic analyses. We will also perform several water casts at night, using the CTD/Niskin rosette on board Atlantis. Scott and Girguis: To address our objectives, it is critical for us to get freshly collected Riftia tubeworms that have been placed in a thermally insulated biobox and sent to the surface as soon after collection as possible (elevators would be fine as long as they were retrieved as soon as they surfaced). Collected tubeworms would be incubated in high-pressure aquaria in the Girguis lab's pressure van for respirometry experiments. After they reach steady-state, they will be subsampled for tissues, enzyme assays, and symbiont preparations (we will need some bench space in one of the ship's labs). Symbiont metabolism will be interrogated via 14C-labelling experiments (we will need to use a radioisotope van and have submitted our radioactive materials use request to WHOI). | |
Additional Info | |
| Pre-cruise Planning Meeting: Teleconference | |
| Meeting Notes: Sievert/Taylor are at WHOI | |
| Media personnel on board: not sure yet | |
Stations: | |
| Station 1 Distance: null Days: null Latitude: 9° 50.0’ N Longitude: 104° 18.0’ W | |
Supporting documentation: | |
| »Survey_sites_and_transit_to_EPR.jpg | |
| »14_MB_Survey_sites_AT26-23_EPR_WPT.pdf | |
| »Ismash_specifications-1.pdf | |
| »Pre-Cruise_Meeting_Agenda_AT26-23_Sievert_Taylor_Scott_White.docx | |
| »Survey_sites_and_transit_to_EPR.jpg | |
| »stefan_dyksma_Rad_Auth.pdf | |
| »BerthingPLAN_AT26-23.pdf | |
| »KS09102014.pdf | |
Funding |
| Funding Agency: NSF | |
| Grant or contract number: IOS-1257532, OCE-1131095 |
Scientific Instrumentation for R/V Atlantis |
Shipboard Equipment | |||||||||||
| Sippican XBT System (Mark 21) | |||||||||||
| Deionized Water System | |||||||||||
| Relay Transponder for Wire Use | |||||||||||
| Transponder Navigation - Sonardyne USBL | |||||||||||
| Navigation - Heading | |||||||||||
| Multibeam | |||||||||||
| Fume Hood | |||||||||||
| Navigation - Position | |||||||||||
Shipboard Communication | |||||||||||
| Basic Internet access via HiSeasNet | |||||||||||
| Is there a need to receive data from shore on a regular basis? | |||||||||||
| Is there a need to transfer data to shore on a regular basis? | |||||||||||
| Is there an expectation to use Skype or any other real-time video conference program? | |||||||||||
CTD/Water Sampling | |||||||||||
| 911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors | |||||||||||
| SBE43 oxygen sensor | |||||||||||
| Wet Labs FLNTURTD Combination Flourometer and Turbidity Sensor | |||||||||||
Critical CTD Sensors | |||||||||||
Sample Storage | |||||||||||
| Climate Controlled Walk-in | |||||||||||
| Freezer -70°C 3.2 cu. ft. ea. | |||||||||||
| Freezer -70°C 25 cu. ft. | |||||||||||
| Refrigerator 8.6 cu. ft. | |||||||||||
| Storage Notes: Girguis/Scott: It would be helpful to have a climate controlled space at 15C with bench space and room for two scientists to work. Let us know if this is possible. | |||||||||||
Navigation | |||||||||||
| Will you be using Long Base Line (LBL) navigation? | no | ||||||||||
| How many nets? | null | ||||||||||
| How many tansponders? | null | ||||||||||
| Will you be using Ultra-short baseline (USBL) navigation? | no | ||||||||||
Navigation | |||||||||||
| GPS | |||||||||||
| USBL | |||||||||||
| Navigation Notes: | |||||||||||
Winches | |||||||||||
| CTD Winch with .322" Electro-mechanical wire | |||||||||||
| Hydro Winch with .25" hydro wire | |||||||||||
| Winch Notes: | |||||||||||
Wire use and application | |||||||||||
| Hydro Winch with .25" hydro wire | |||||||||||
| CTD Winch with .322" Electro-mechanical wire | |||||||||||
| Winch Notes: | |||||||||||
Standard Oceanographic Cables | |||||||||||
| |||||||||||
Portable Vans | |||||||||||
| Isotope Van | |||||||||||
| Chemical Storage Van | |||||||||||
| Science Van 1 | |||
| Type/size: Girguis lab pressure van | Location: | ||
| Water: | Power: | ||
| Science Van 2 | |||
| Type/size: | Location: 01 deck next to the emergency generator room | ||
| Water: we need clean seawater, as well as freshwater in the pressure van | Power:480VDC (three phase); two circuits @ 60A each | ||
Specialized Deck Equipment
| Mooring Deployment/Recovery Equipment Required: no | Type: |
| Cruise Specific Science Winch Required: no | Type: |
| Nets Required: no | Type: |
Over the Side Equipment
Special Requirements
| Elecrical Power: yes | Identify See Girguis Lab pressure van above |
| Equipment Handling: no | Identify: |
| Inter/intraship Communications: no | Identify: |
| Science Stowage: yes | Identify: We would like to consider leaving the pressure van on the ship until we reach a US port. |
| Water: yes | Identify: See pressure van |
Additional Cruise Items/Activities
Hazardous Material
Radioactive Material
Additional Information
Sievert/Taylor/Rich/White: We will be perfoming water casts using the ship-board CTD/Rosette system
Scientific Instrumentation for HOV Alvin |
Site Survey | |||||||
| Will you provide detailed charts of the work area(s)? | no | ||||||
| If no, willl you need R/V Atlantis to generate maps of the work area(s)? | no | ||||||
| Will you need post-dive maps of the work area generated? | no | ||||||
Navigation | |||||||
| Will you be using Long Base Line (LBL) navigation? | no | ||||||
| How many nets? | 0 | ||||||
| How many tansponders? | 0 | ||||||
| Will you be using Ultra-short baseline (USBL) navigation for other than Alvin operations? | yes | ||||||
Vehicle Equipment | |||||||
| CTD | |||||||
| Search sonar | |||||||
| Bio collection boxes: 12 x 12 x 24 | |||||||
| High temperature probe (0-480°C) | |||||||
| Low temperature probe | |||||||
| Bio collection boxes: 12 x 12 x 12 | |||||||
| Large capacity slurp samplers: multi-chamber | |||||||
| Small capacity slurp samplers | |||||||
| Push corers (12-pack rack) | |||||||
| Scoop nets | |||||||
Elevators | |||||||
| Will you be using elevators to transport samples to the surface? | yes | ||||||
| If yes, how many would you anticipate? | 6 (G/S), 10 (S/T/R) | ||||||
Science Supplied Equipment | |||||||
| Are you supplying equipment to be used on HOV Alvin | yes | ||||||
| Has this equipment been used on Alvin before? | no | ||||||
| Please give a brief description of the equipment, its intended purpose, the cruise # it was last used on if any and its deployment method.
Sievert/Taylor/Rich: We are going to use a a number of instruments, msot of which have been used with Alvin: 1. We will be using Jeff Seewald's isobaric samplers, which have been used frequently with Alvin 2. We will be using chemical in situ sensors developed by Nadine LeBris, these are self-contained units that have been frequently used with Alvin (last time on AT15-38) 3. A McLane in situ large volume pump is going to be deployed as an elevator. Alvin will need to move the pump to the deployment site and also release it after filtration is finished (6-8 hours) 4. Vent-TSSID: This is a newly devleoped isntrument that has not yet been used with Alvin. The pressure senstive components will be pressure tested according to Alvin specificications. Fred Thwaites who is engeering the isntruemnt has been in touch with Alvin group. Girguis/Scott: We may possibly be gringing in situ blenders to preserve tubeworms on the seafloor. They are not pressurized. | |||||||
| Does this equipment use an external pressure housing? | no | ||||||
| Has the pressure housing been tested per Alvin Pressure Test requirements? | no | ||||||
| Pressure housing schematic with dimensions and include air and water weights. | |||||||
| Air weight of this equipment? | 12 | ||||||
| Water weight of this equipment? | null|||||||
| Does the equipment require a power interface from Alvin? | no | ||||||
| Voltage required null | |||||||
| Power required | null|||||||
| Data Interface | null|||||||
| Does the equipment meet the electrical requirements of the Alvin User Manual? | no | ||||||
| Please provide power and comms specifics, wiring diagram, pinout, and wiring harness in advance of the cruise. | |||||||
| Does this equipment require hydraulic inputs from the vehicle? | yes | ||||||
| |||||||
|
Hydraulic schematic of the equipment requirements.
»ismash_specifications-1.pdf | |||||||
| Does this equipment require manipulation? | yes | ||||||
| If yes, please describe how the equipment is to be manipulated:
Girguis/Scott: PIlot needs to open and close lid. Very minor. Specifications are included in the diagram uploaded for the hydraulic schematic. Device has been successfully used with the JASON. Sievert/Taylor: LVP and Vent-TSSID need to be moved by Alvin, intake wand needs to be placed in opening of vent, instrument weights need to be released at end of operation | |||||||
| Will this equipment be deployed off the vehicle? | no | ||||||
| If yes, please describe how the equipment is intended for deployment: | |||||||
| If yes, will the equipment be disconnected from the vehicle and left in situ? | no | ||||||
| How long will the deployment be? | |||||||
| Will the equipment be recovered by the same vehicle? | no | ||||||
| Do you intend to recover any other previously deployed equipment? | no | ||||||
| If yes, please describe this equipment: | |||||||
| Does this equipment use any glass spheres for either buoyancy or as pressure housings? | no | ||||||
| Does the equipment have an associated computer or control panel for remote operation from the personnel sphere? | no | ||||||
| Has the computer or control panel been gas tested for use in the personnel sphere? | no | ||||||
Hazardous Material | |||||||
| Will hazardous material be utilized? | no | ||||||
| Describe deployment method and quantity: | |||||||
Additional Information | |||||||
| Brief operations description or comments: | |||||||
| Navy Clearance: Granted 11/04/14: WITHIN 10 NM RADIUS OF 09-50N 104-17W DURING PERIOD 020000T THRU 262400T NOV 14. WITHIN 10 NM RADIUS OF 09-39N 104-14W DURING PERIOD 020000T THRU 262400T NOV 14. WITHIN 10 NM RADIUS OF 09-54N 104-15W DURING PERIOD 020000T THRU 262400T NOV 14. | |||||||
Checklist & Notes |
Checklist | |
| U.S. Customs Form: | yes |
| Diplomatic Clearance: | yes |
| Date Submitted: | Feb 27, 2014 |
| Date Approved: | Sep 18, 2014 |
| Agent Information: | |
Mexico (Manzanillo)Primary Agent:Vasile Tudoran Transport 819 Ohio Ave. Long Beach, CA 90804 Contact: Vasile Tudoran Phone: (562) 882-5590 Fax: (562) 434-9800 Email : vtudoran@aol.com Subagent: Master R/V Ship Name Attn: Scientist's Name IN TRANSIT MANZANILLO MEXICO c/o Transmex Shipping, S de R.L de C.V. Colhuas No.83 Centro Manzanillo, Colima MEXICO Contact: Daniel Arias Romo Phone: 011 52 314 33 2 20 65 / 33 2 80 22 Fax: 011 52 55 10 842917 Cell: 011 52 1 314 12 1 48 42 Email: danielromo2007@gmail.com Note: Agent and WHOI contacts should be copied on all communications. It is requested that shipment information of any equipment be communicated to the Agent and WHOI contacts Costa RicaMaster R/V Atlantis | |
| Countries: | |
| Mexico and Costa Rica. All work done outside EEZs. | |
| Notes: | |
| Forms to Eric by Oct 17. San Fran load port. | |
| Isotope Use Approval: | yes |
| Isotope Notes: | |
| Stefan Dystick. | |
| SCUBA Diving: | no |
Checklist | |
| SSSG Tech: | |
| Dave Sims, Allison Heater | |
| Pre load BAE San Fran Oct 20 and 21. | |
| Puerto Caldera will be dock on arrival Nov 26 to off load lab van and science samples and equipment. Caldera address is: INCOOP / Puerto Caldera, Costa Rica. Container Terminal. Leave container terminal out of the google search. | |