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Woods Hole Oceanographic Institution


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Publications
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»Mar Ecol 2000
»Appl Env Microb 2000
»FEMS Microb Ecol 2001
»Appl Env Microb 2005
»Deep-Sea Res 2006
»Env Micro 2006
»Aquat Microb Ecol 2006, 1
»Aquat Microb Ecol 2006, 2
»GCA 2009
»FEMS Microb Ecol 2009
»Aquat Microb Ecol 2010
»GCA 2010
»Org Geochem 2010
»Env Micro 2010
»L & O 2011
»ES&T 2011
»GCA 2011
»Env Res Letters 2011
»Org Geochem 2012
»Geomicro accepted


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K. Longnecker and A.-L. Reysenbach, Expansion of the geographic distribution of a novel lineage of ε -Proteobacteria to a hydrothermal vent site on the Southern East Pacific Rise, FEMS Microbiology Ecology, 2001, 35 (3):287-293.

The diversity associated with a microbial mat sample collected from a deep-sea hydrothermal vent on the Southern East Pacific Rise was determined using a molecular phylogenetic approach based on the comparison of sequences from the small subunit ribosomal RNA gene (16S rDNA). The DNA was extracted from the sample and the 16S rDNA was amplified by PCR. Sixteen different phylotypes were identified by restriction fragment length polymorphism analysis; four phylotypes were later identified as putative chimeras. Analysis of the 16S rDNA sequences placed all the phylotypes within the Proteobacteria. The majority of the sequences (98%) were most closely related to a new clade of ε-Proteobacteria that were initially identified from an in situ growth chamber deployed on a deep-sea hydrothermal vent on the Mid-Atlantic Ridge in 1995. The similarity between phylotypes identified from Atlantic and Pacific deep-sea hydrothermal vent sites indicates that this new clade of Proteobacteria may be endemic to and widely distributed among deep-sea hydrothermal vents.

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