Woods Hole Oceanographic Institution
Cruise Planning Synopsis
AT37-12 Sievert "In situ Rates" | |
Ship | |
| R/V Atlantis | |
Vehicles | |
| HOV Alvin | |
Cruise Party | |
| Jeremy Rich: Principal Investigator University of Maine 93 Clarks Cove Rd Walpole, ME USA 04573 +1 207 563 8302 jeremy.rich@maine.edu Stefan Sievert: Chief Scientist, Principal Investigator Woods Hole Oceanographic Institution Watson Building 207, MS#52 Woods Hole, Ma. USA 02543 +1 508 289 2305 ssievert@whoi.edu Craig Taylor: Principal Investigator Woods Hole Oceanographic Institution Watson Building 209, MS#52 Woods Hole, Ma. USA 02543 +1 508 289 2354 ctaylor@whoi.edu |
Departure: Apr 24, 2017 | |
| Manzanillo Mexico | |
Arrival: May 14, 2017 | |
| Puntarenas Costa Rica | |
Operations Area | |
| 9 North Integrated Study Site | |
| Lat/Lon: 9° 50.0′ N / 104° 18.0′ W | |
| Depth Range: 2500 / 2600 | |
| Will the vessel be operating within 200 NM of a foreign country? | no |
Science Objectives | |
Knowledge of the in situ metabolism of microorganisms carrying out CO2-fixation at deep-sea hydrothermal vents is very limited. Particularly lacking are studies measuring rates of autotrophic carbon fixation in situ, which is a measurement ultimately needed to constrain production in these ecosystems. Although recent data suggests that nitrate reduction either to N2 (denitrification) or to NH4+ (dissimilatory reduction of nitrate to ammonium, DNRA) might be responsible for a significant fraction of chemoautotrophic production, NO3--reduction rates have never been measured in situ at hydrothermal vents. We hypothesize that chemoautrophic growth is strongly coupled to nitrate respiration in vent microbial communities. As part of this cruise, we are going to use a recently developed robotic micro-laboratory (Vent-SID) to measure rates of chemoautotrophic production and to determine the relative importance of oxygen and nitrate in driving chemosynthesis at hydrothermal vents at both in situ pressures and temperatures and to tackle the following currently unresolved science objectives: 1) obtain in situ rates of chemoautotrophic carbon fixation, 2) obtain in situ nitrate reduction rate measurements, and 3) directly correlate the measurement of these processes with the expression of key genes involved in carbon and energy metabolism. | |
Science Activities | |
The cruise has 12 dives with DSV Alvin, to allow time for deployment and collection of experiments and for sampling of discrete vents along the axis of the 9°46’N to 9°53’N segment of the East Pacific Rise (EPR). We will focus our activities at Crab Spa, a diffuse flow vent site near Tica, and deploy experiments and sampling equipment, including the Vent-SID and a large volume pump (LVP). During the course of the cruise we will perform several deployments of the Vent-TSSID, as well as collect biomass from fluids and biofilms (deployment/recover experimental microbial colonizers) by utilizing the LVP. Both the Vent-SID and the LVP will be deployed as an elevator. It is planned to deploy the instruments and bring them close to the deployment site the night before a dive, and then to position the instruments at the site with Alvin the next day. Alvin will be releasing the instrument either on the same dive or any subsequent dive, after which the instrument will be picked up at the surface. Furthermore, we will carry out additional opportunistic sampling of other diffuse-flow vents. Fluid samples for chemical analyses will be collected at each deployment site using isobaric gas-tight samplers, which will also be used to sample fluids from the focus site for the shipboard incubations. Finally, we will deploy/recover experimental microbial colonizers to collect microbial biofilms, as well as collect Riftia for subsequent ‘omic analyses. We are also planning to perform several water casts at night, using the CTD/Niskin rosette on board Atlantis. | |
Additional Info | |
| Pre-cruise Planning Meeting: Visit WHOI | |
| Meeting Notes: Sievert/Taylor are at WHOI | |
| Media personnel on board: not sure yet | |
Stations: | |
| Station 1 Distance: null Days: null Latitude: 9° 50.0’ N Longitude: 104° 18.0’ W Station 2 Distance: null Days: 12 Latitude: 9° 50.0’ N Longitude: 104° 18.0’ W | |
Supporting documentation: | |
| »Vent-SID.jpg | |
| Notes: Q&A
From the Chief Mate Q: “It sounds like night ops would consist of deploying and recovering elevators (maybe 1 per night) and CTD's. Are there any other night operations planned, and if so, what ship's equipment would they use?” A: No other night ops planned. For the CTD we might move away a couple of hours from the dive site. We probably will have night ops every night, but probably not elevators every night. From SSSG@Atlantis Q: “Stefan - tell me what will be different about this cruise than your other cruises in the past?” A: It will be very similar to previous cruises, especially AT26-23, during which we used the Vent-SID. | |
Funding |
| Funding Agency: NSF | |
| Grant or contract number: OCE-1559198 |
Scientific Instrumentation for R/V Atlantis |
Shipboard Equipment | |||||||||||
| Deionized Water System | |||||||||||
| Relay Transponder for Wire Use | |||||||||||
| 12 kHz Pinger for Wire Use | |||||||||||
| Transponder Navigation - Sonardyne USBL | |||||||||||
| Navigation - Heading | |||||||||||
| Multibeam | |||||||||||
| Fume Hood | |||||||||||
| Navigation - Position | |||||||||||
Shipboard Communication | |||||||||||
| Basic Internet access via HiSeasNet | |||||||||||
| Is there a need to receive data from shore on a regular basis? | |||||||||||
| Is there a need to transfer data to shore on a regular basis? | |||||||||||
| Is there an expectation to use Skype or any other real-time video conference program? | |||||||||||
CTD/Water Sampling | |||||||||||
| 911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors | |||||||||||
| SBE43 oxygen sensor | |||||||||||
| Wet Labs FLNTURTD Combination Flourometer and Turbidity Sensor | |||||||||||
Critical CTD Sensors | |||||||||||
Sample Storage | |||||||||||
| Climate Controlled Walk-in | |||||||||||
| Freezer -70°C 3.2 cu. ft. ea. | |||||||||||
| Freezer -70°C 25 cu. ft. | |||||||||||
| Refrigerator 8.6 cu. ft. | |||||||||||
| Storage Notes: Girguis/Scott: It would be helpful to have a climate controlled space at 15C with bench space and room for two scientists to work. Let us know if this is possible. | |||||||||||
Navigation | |||||||||||
| Will you be using Long Base Line (LBL) navigation? | no | ||||||||||
| How many nets? | null | ||||||||||
| How many tansponders? | null | ||||||||||
| Will you be using Ultra-short baseline (USBL) navigation? | no | ||||||||||
Navigation | |||||||||||
| GPS | |||||||||||
| USBL | |||||||||||
| Navigation Notes: | |||||||||||
Winches | |||||||||||
| CTD Winch with .322" Electro-mechanical wire | |||||||||||
| Hydro Winch with .25" hydro wire | |||||||||||
| Winch Notes: | |||||||||||
Wire use and application | |||||||||||
| Hydro Winch with .25" hydro wire | |||||||||||
| CTD Winch with .322" Electro-mechanical wire | |||||||||||
| Winch Notes: | |||||||||||
Standard Oceanographic Cables | |||||||||||
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Portable Vans | |||||||||||
| Chemical Storage Van | |||||||||||
Specialized Deck Equipment | |||||||||||
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Over the Side Equipment | |||||||||||
| Will you be bringing any equipment (winches, blocks, etc.) that lowers instruments over the side? | no | ||||||||||
Special Requirements | |||||||||||
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Additional Cruise Items/Activities | |||||||||||
| Explosive Devices: | no | ||||||||||
| Portable Air Compressors: | no | ||||||||||
| Flammable Gases: | yes | ||||||||||
| Small Boat Operations: | no | ||||||||||
| SCUBA Diving Operations: | no | ||||||||||
Hazardous Material | |||||||||||
| Will hazardous material be utilized? | yes | ||||||||||
| Describe deployment method and quantity: | |||||||||||
Radioactive Material | |||||||||||
| Radioiosotopes: | yes | ||||||||||
Additional Information | |||||||||||
| Is night time work anticipated on this cruise? | yes | ||||||||||
| Specialized tech support (Seabeam, coring, other): Sievert/Taylor/Rich/White: we will be perfoming water casts using the ship-board CTD/Rosette system, we will also deploy elevators the night before an Alvin dive | |||||||||||
| Other required equipment and special needs: | |||||||||||
Scientific Instrumentation for HOV Alvin |
Site Survey | |
| Will you provide detailed charts of the work area(s)? | no |
| If no, willl you need R/V Atlantis to generate maps of the work area(s)? | no |
| Will you need post-dive maps of the work area generated? | no |
Navigation | |
| Will you be using Long Base Line (LBL) navigation? | no |
| How many nets? | 0 |
| How many tansponders? | 0 |
| Will you be using Ultra-short baseline (USBL) navigation for other than Alvin operations? | yes |
Vehicle Equipment | |
| CTD | |
| Search sonar | |
| Bio collection boxes: 12 x 12 x 24 | |
| Bio collection boxes: 12 x 12 x 12 | |
| Large capacity slurp samplers: multi-chamber | |
| Small capacity slurp samplers | |
| Scoop nets | |
| Major (dual) pair water sampler(s) with ICL temperature probles | |
| Special micro-bio box 12" x 18" x 12" | |
Elevators | |
| Will you be using elevators to transport samples to the surface? | yes |
| If yes, how many would you anticipate? | Vent-SID: 4 LVP: 2-4 |
Science Supplied Equipment | |
| Are you supplying equipment to be used on HOV Alvin | yes |
| Has this equipment been used on Alvin before? | yes |
| Please give a brief description of the equipment, its intended purpose, the cruise # it was last used on if any and its deployment method.
We are going to use a a number of instruments, most of which have been used with Alvin: 1. We will be using chemical in situ sensors developed by Nadine LeBris, these are self-contained units that have been frequently used with Alvin (last time on AT15-38) 3. A McLane in situ large volume pump is going to be deployed as an elevator. Alvin will need to move the pump to the deployment site and also release it after filtration is finished (6-8 hours) 4. Vent-SID: This is a newly developed isntrument that has been used with Alvin during AT26-23. The pressure senstive components have been pressure tested according to Alvin specificications. Fred Thwaites, who is the main engineer of the instrument, has been in touch with Alvin group, and so has Steve Faluotico, who is implementing an ICL to communicate to the instrument from the sphere. | |
| Does this equipment use an external pressure housing? | yes |
| Has the pressure housing been tested per Alvin Pressure Test requirements? | yes |
| Pressure housing schematic with dimensions and include air and water weights.
»Pressure_Cert_14-174.pdf »Pressure_Test_14-175_10-02-2014_Acoustic_Report.pdf | |
| Air weight of this equipment? | 1,200 (Vent-SID); 300 (LVP) |
| Water weight of this equipment? | 480 (Vent-SID); 95 (LVP) |
| Does the equipment require a power interface from Alvin? | yes |
| Voltage required | I am not sure here. We are in the process of implementing an ICL to commnicate with the Vent-SID. Steve Faluotico is the person working on it and he has been in touch with Alvin group (Anthony Tarantino) |
| Power required | |
| Data Interface | Other |
| Does the equipment meet the electrical requirements of the Alvin User Manual? | yes |
| Please provide power and comms specifics, wiring diagram, pinout, and wiring harness in advance of the cruise. | |
| Does this equipment require hydraulic inputs from the vehicle? | no |
| Hydraulic schematic of the equipment requirements. | |
| Does this equipment require manipulation? | yes |
| If yes, please describe how the equipment is to be manipulated:
LVP and Vent-SID need to be moved by Alvin, intake wand needs to be placed in opening of vent, instrument weights need to be released at end of operation | |
| Will this equipment be deployed off the vehicle? | no |
| If yes, please describe how the equipment is intended for deployment: | |
| If yes, will the equipment be disconnected from the vehicle and left in situ? | no |
| How long will the deployment be? | |
| Will the equipment be recovered by the same vehicle? | no |
| Do you intend to recover any other previously deployed equipment? | no |
| If yes, please describe this equipment: | |
| Does this equipment use any glass spheres for either buoyancy or as pressure housings? | yes |
| Does the equipment have an associated computer or control panel for remote operation from the personnel sphere? | yes |
| Has the computer or control panel been gas tested for use in the personnel sphere? | yes |
Hazardous Material | |
| Will hazardous material be utilized? | no |
| Describe deployment method and quantity: | |
Additional Information | |
| Brief operations description or comments: | |
| Navy Clearance: Granted 4/20/2017: WITHIN 150 NM RADIUS OF 09-50N 104-18W DURING PERIOD 240000T APR THRU 142400T MAY 17 | |
Checklist & Notes |
Checklist | |
| U.S. Customs Form: | no |
| Diplomatic Clearance: | no |
| Date Submitted: | |
| Date Approved: | |
| Agent Information: | |
| In COSTA RICA (preloading only) Contact: Vasile Tudoran Phone: (562) 882-5590 Fax: (562) 434-9800 Email: vtudoran@aol.com Local Address for Large Shipments: Master R/V Ship Name Attn: Scientist's Name IN TRANSIT SERMAR SA Aptdo 76 Puntarenas, Costa Rica Attn: Manuel Fernandez Phone: 011-506-2661-1529 Fax: 011-506-2661-2779 In Mexico: Primary Agent:
Vasile Tudoran Transport 819 Ohio Ave. Long Beach, CA 90804 Contact: Vasile Tudoran Phone: (562) 882-5590 Fax: (562) 434-9800 Email : vtudoran@aol.com Subagent: Master R/V Ship Name Attn: Scientist's Name IN TRANSIT MANZANILLO MEXICO c/o Transmex Shipping, S de R.L de C.V.
Constitucion No.27-D Interior
Colonia Burocrata
Manzanillo, Colima
C.P.28250
MEXICO
Tel.314 33 2 20 65
Cell: 314 121 48 42
Nextel: 1206098 / ID-72*153248-*
Email: danielromo2007@gmail.com
| |
| Countries: | |
| Mexico Cost Rica | |
| Notes: | |
| Vent-SID Components are being shipped to Costa Rica to be loaded during the Luther mobilization: 1. Vent-SID Top frame box (56" * 41" * 93"), 1,200 lbs 2. Vent-SID Bottom frame box (69" * 69" *41), ~300 lbs | |
| Isotope Use Approval: | no |
| Isotope Notes: | |
| SCUBA Diving: | no |
Checklist | |
| SSSG Tech: | |
| Allison, Catie | |
| Vent-SID can be kept on the outer decks | |