Woods Hole Oceanographic Institution

Cruise Planning Questionnaire



R/V Atlantis


Cruise Party

Patricia Yager: Chief Scientist, Principal Investigator
University of Georgia - Department of Marine Sciences 220 Marine Sciences Bldg. Athens, Georgia USA 30602-3636
+1 706 542 6824

Departure: Bridgetown Barbados on Jun 23, 2012

Arrival: Bridgetown Barbados on Jul 9, 2012

Mobilization Date: Jun 21, 2012

Demobilization Date: Jul 11, 2012

Supporting documentation:


Operations Area: Western Tropical North Atlantic

Lat/Lon: 3° 0.0′ N / 50° 0.0′ W

Depth Range: 10 / 4500

Will the vessel be operating within 200 NM of a foreign country? Brazil, French Guiana, Barbados
Are visas or special travel documents required? no

Science objectives

The ANACONDAS project builds on observations made by MANTRA/PIRANA in 2001 and 2003 (RV Knorr and Seward Johnson I cruises to the same region) to address specifically 1) how carbon cycling and sequestration in the western tropical North Atlantic (WTNA) is influenced by the Amazon River through its impact on pelagic ecosystem dynamics and 2) the sensitivity of this ecosystem to anthropogenic climate change.  PIRANA revealed the importance of both riverine and atmospheric inputs for driving the high productivity of the WTNA through N2-fixation, and demonstrated the significance of the region to basin-wide biogeochemistry and C cycling. ANACONDAS will now focus on what drives phytoplankton community succession through the plume, light and nutrient requirements, factors limiting productivity, and the fate of production. These components are critical to understand the role of the plume in the regional C cycle, and to predict its response to climate variability and change.    
  The NSF-funded ANACONDAS project will also serve as a platform for additional measurements  supported by the Gordon and Betty Moore Foundation's Marine Microbiology Initiative.  ROCA (River-Ocean Continuum of the Amazon) brings additional focus on marine microbial community structure and activities, along with high-resolution measurements of organic matter along the river-ocean continuum. 

Science Activities

Water Column Characterization (hydrographic sampling with CTD/Rosette):
Nutrient (NO2, NO2+NO3, PO4, SiO4) concentrations
Chlorophyll a and pigments concentrations
Inorganic carbon (discrete DIC, ALK, underway pCO2)
Organic carbon, nitrogen, phosphorus
Phytoplankton and Diazotroph Abundance (using rosette and also small nets to collect)
Carbon and Nitrogen Fixation by plankton
Kinetic and Physiological Measurements of phytoplankton 
Stable Isotopic Measurements of particulate material
Microbial heterotrophy
Microbial community structure and gene expression
Organic carbon and biomarker characterization

MOCNESS tows for zooplankton
Zooplankton collection for abundance and biomass
Zooplankton grazing and POC flux measurements

Multicorer for deep sea sediment analyses
Solid phase analysis
Pore water chemistry
Isotopic composition (Pb, Th, C)

Other instrumentation over the side:
fast rate repetition fluorometer to measure phytoplankton physiology
Optics Cage - a suite of optical sensors designed to characterize the light and fluorescence field
Spectrial radiometer - also used to characterize the light field and link to satellite measurements
The in-water light field will be characterized with a free-falling 14 channel spectroradiometer 
Two “Carbon Explorers” – autonomous Sounding Oceanographic Lagrangian Observer profilers 
Sediment Trap Studies (using 48h deployments of floating Particle Interceptor Traps; PITs)
Surface water pumps - directly bring large volumes of surface water to the deck of the ship for processing.

Pre-cruise planning meeting: Teleconference


Funding Agency: NSF #OCE 0934095, Gordon and Betty Moore Foundation #2293 / 2928

- added NSF #OCE 0934095 on Feb 1, 2012 1:35 PM by Dr. Patricia L. Yager
- added Gordon and Betty Moore Foundation #2293 / 2928 on Feb 1, 2012 1:35 PM by Dr. Patricia L. Yager

R/V Atlantis

Shipboard Equipment

12 kHz Pinger for Wire Use
ADCP 75 kHz
Bathymetry System 12 kHz
Deionized Water System
Navigation - Position
Science Underway Seawater System

Shipboard Communication

Basic Internet access via HiSeasNet
Is there a need to receive data from shore on a regular basis?
Is there a need to transfer data to shore on a regular basis?

CTD/Water Sampling

911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors
Biospherical underwater PAR (1000m depth limit) with reference Surface PAR
SBE43 oxygen sensor
Seapoint STM turbidity sensor
Wet Labs C*Star transmissometer (660nm wavelength)
Wet Labs ECO-AFL fluorometer

Critical CTD Sensors: 

Hydrographic Analysis Equipment

Dissolved Oxygen Titration System (Brinkmann Titrator)
Oxygen Sample Bottles (available in 150 ml sizes)
Salt Bottles (2 cases of 125 ml provided)

MET Sensors

Air temperature
Short Wave Solar Radiation
Wind speed and direction

Sediment Sampling


Sample Storage

Climate Controlled Walk-in
Freezer -70°C 25 cu. ft.
Freezer -70°C 3.2 cu. ft. ea.
Refrigerator 8.6 cu. ft.
Scientific Walk-in Freezer

Storage Notes:


Will you be using Long Base Line (LBL) navigation? no

Will you be using Ultra-short baseline (USBL) navigation for other than Alvin operations? no



Navigation Notes: We will need good maps of the coastal zone of Brazil - especially good depth maps since we will be going near the mouth of the river.  We may be able to get these from our Brazilian collaborators, but we should try to get them ourselves.


CTD Winch with .322" Electro-mechanical wire
Hydro Winch with .25" hydro wire
Other Portable Winch

Winch Notes:

Wire use and application

Hydro Winch with .25" hydro wire

Wire Notes: Spectral radiometer (<100 lbs in air, <50 lbs in water), 100 m, hydrowire
FRRF (<200 lbs in air, <100 lbs in water), 100 m, hydrowire
Optics Cage (<200 lbs in air, <100 lbs in water), 100 m, hydrowire
Slip ring required? no Number of conductors: 
Non-standard wire required? no Type: 
Traction winch required? no Describe: 

Portable Vans

Chemical Storage Van
Cold Storage Van
Isotope Van

Other Science Vans:

Other Science Vans:
Science Van 1
Type/size: Stable isotope van for 15N, 13C Location:
Water: no Power:yes
Science Van 2
Type/size: Radioisotope Van #2 (for 3H) Location:
Water: no Power:yes

Specialized Deck Equipment

Mooring Deployment/Recovery Equipment Required: yes Type: floating sed trap, argos float
Cruise Specific Science Winch Required: no Type: 
Nets Required: yes Type: 200 um MOCNESS, ring nets

Over the Side Equipment

Will you be bringing any equipment (winches, blocks, etc.) that lowers instruments over the side? no

Special Requirements

Elecrical Power: yes Identify: clean power line for instruments in analytical lab
Equipment Handling: no Identify: 
Inter/intraship Communications: no Identify: 
Science Stowage: yes Identify: Science cargo van?
Water: yes Identify: Milliq (deionized water) is a critical need

Additional Cruise Items/Activities

Explosive Devices: no
Portable Air Compressors: no
Flammable Gases: no
Small Boat Operations: no
SCUBA Diving Operations: no

Hazardous Material

Will hazardous material be utilized? yes

Radioactive Material

Radioiosotopes: yes

Additional Information

Is night time work anticipated on this cruise? yes

Specialized tech support (Seabeam, coring, other): 24-hour operations (CTD, MOCNESS, Multicore) - no extra techs needed, just standard Marine Techs on 12h watches.

Other required equipment and special needs: This cruise will only go if we get permission to sample in Brazil EEZ.  The departure date may slip a few weeks in order to make this happen.  Timeline for decisions will be established between Yager, WHOI, and UNOLS.
Date Submitted: Feb 1, 2012 2:32 PM by Dr. Patricia L. Yager