Woods Hole Oceanographic Institution
Cruise Planning Questionnaire
Guaymas Basin 2016
Ship
R/V AtlantisVehicles
AUV SentryCruise Party
Andreas Teske: Chief Scientist, Principal InvestigatorUniversity of North Carolina at Chapel Hill Marine Sciences, CB 3300 Chapel Hill, NC USA 27599
+1 919 843 2463
teske@email.unc.edu
Departure: Manzanillo on Dec 9, 2016
Arrival: Manzanillo on Dec 27, 2016
Mobilization Date: Dec 7, 2016
Demobilization Date: Dec 29, 2016
Supporting documentation:
»GuaymasSentryRationalePDF_copy.pdf»Cruise_schedule_December_2016PDF.pdf
»Cruise_Track_Manzanillo_-_Guaymas_Basin_-_Manzanillo_copy.pdf
Operations Area: Southern Guaymas Basin
Lat/Lon:
Depth Range: 1900 / 2050
Will the vessel be operating within 200 NM of a foreign country? Mexico
Are visas or special travel documents required? no
Science objectives
This integrated biogeochemical and microbiological research program will explore the pathways
of and environmental controls on the consumption and production of methane, other alkanes,
DIC, LMW organic acids and sedimentary organic matter that fuel the Guaymas sedimentary microbial ecosystem. The hydrothermal sediments of Guaymas Basin provide a spatially compact, high-activity location for investigating novel modes of methane cycling and carbon assimilation into microbial biomass. In the case of anaerobic methane oxidation, the high temperature and pressure tolerance
of Guaymas Basin methane-oxidizing microbial communities, and their potential to uncouple
from the dominant electron acceptor sulfate, vastly increase the predicted subsurface habitat
space and biogeochemical role for anaerobic microbial methanotrophy in global deep subsurface diagenesis. Further, microbial methane production and oxidation interlocks with S and N transformations, which we will explore on the organism and process level in hydrothermal sediment microbial communities and mats of Guaymas Basin. In general, our first-order research tasks (rate measurements, radiotracer incorporation studies, genomes, in situ microgradients) will define the key microbial capabilities, pathways and processes that mediate chemical exchange between the subsurface hydrothermal/seep environment and the deep ocean waters.
Science Activities
In the proposed project, we will investigate genomic potential, physiological capabilities and biogeochemical roles of key uncultured organisms from Guaymas sediments, especially the high-temperature anaerobic methane oxidizers that occur specifically in hydrothermally active sediments; for this purpose, sediment cores from hydrothermal hot spots [to be determined with the Alvin heatflow probe] will be collected with HOV Alvin. We will focus on the role of methane-oxidizing archaea and other Guaymas microbiota in C transformations, but also explore their potential involvement in S and N transformations. First-order research topics include quantifying anaerobic methane oxidation under high T, P and in situ methane concentration, and with alternate electron acceptors; sulfate and sulfur-dependent microbial pathways and isotopic signatures under these conditions; and nitrogen transformations in methane-oxidizing microbial communities, hydrothermal mats and sediments. To complement these microbial and biogeochemical studies, we are planning to employ the AUV Sentry, build and operated by the Woods Hole Oceanographic Institution, for a mapping and photomosaic survey of the Southern Guaymas Trench and its adjacent regions. This survey should enlarge the commonly visited microbial sampling area, which is needlessly limited to a small area in the southern Guaymas Basin.
Pre-cruise planning meeting: Teleconference/Visit WHOI
I will be at WHOI on Friday, August 26Stations:
Station 1Distance: null
Days: 13
Latitude: 27.0 N
Longitude: 111.25 W
Station 2
Distance: 650
Days: 13
Latitude: 27.0 N
Longitude: 111.25 W
Funding Agency: NSF #1357238
- added NSF #1357238 on Aug 23, 2016 8:52 PM by
<Could not load the preview of Funding data >
R/V Atlantis
Shipboard Equipment
Deionized Water SystemScience Underway Seawater System
Fume Hood
Shipboard Communication
Basic Internet access via HiSeasNetCTD/Water Sampling
911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensorsSBE43 oxygen sensor
Critical CTD Sensors:
Sample Storage
Climate Controlled Walk-inFreezer -70°C 25 cu. ft.
Refrigerator 8.6 cu. ft.
Storage Notes: Sediments are either kept alive at +4C in the coldroom for shorebased work, or are frozen at -80C for DNA/RNA shorebased work. Similar for sediment porewater; DIC samples are usually frozen, methane can be stored at RT, and sulfide/sulfate can be stored refrigerated at 4C. Fixed microbial cells are usually stored at -20C.
Navigation
Will you be using Long Base Line (LBL) navigation? no
Will you be using Ultra-short baseline (USBL) navigation for other than Alvin operations? no
Navigation
USBLNavigation Notes: By my respone to the site survey question, I mean that ROV Sentry will generate new and better maps of the southern Guaymas Basin area; see the attached pdf "GuaymasSentryRationale". The currently available maps for this area are quite sketchy, and every expedition is really on its own.
Alvin has used its own navigation system to find dive targets in Guaymas Basin, and this has worked quite well on previous cruises.
Winches
CTD Winch with .322" Electro-mechanical wireWinch Notes: Essentially, the standard CTD winch will be needed, and Atlantis's winch system to release and recover SENTRY; but here I don't know any details.
Wire Notes:
Slip ring required? no | Number of conductors: |
Non-standard wire required? no | Type: |
Traction winch required? no | Describe: |
Portable Vans 
Isotope VanOther Science Vans:
Specialized Deck Equipment
Mooring Deployment/Recovery Equipment Required: no | Type: |
Cruise Specific Science Winch Required: no | Type: |
Nets Required: no | Type: |
Over the Side Equipment
Will you be bringing any equipment (winches, blocks, etc.) that lowers instruments over the side? noSpecial Requirements
Elecrical Power: no | Identify: |
Equipment Handling: no | Identify: |
Inter/intraship Communications: no | Identify: |
Science Stowage: yes | Identify: pre-loading of some gear in Newport Oct 6-8 |
Water: yes | Identify: deionized water for fancy microbiologists |
Additional Cruise Items/Activities
Explosive Devices: no Portable Air Compressors: no Flammable Gases: no |
Small Boat Operations: no SCUBA Diving Operations: no |
Hazardous Material
Will hazardous material be utilized? yes
Radioactive Material
Radioiosotopes: yesAdditional Information
Is night time work anticipated on this cruise? yes
Specialized tech support (Seabeam, coring, other): Sentry is operated during the night so that it does not interfere with Alvin dives during the day; bunk space for Sentry personnel has been accommodated.
Other required equipment and special needs:
AUV Sentry
Site Survey
Will you provide detailed charts of the work area(s)? yesCurrent Chart(s):
»MapofSamplingAreaPDF.pdf
If no, will you need Sentry to generate maps of the work area(s)? no
Will you need post-dive maps of the work area generated? no
Navigation
Will you be using Long Base Line (LBL) navigation? no
Will you be using Ultra-short baseline (USBL) navigation? no
Will you be using Doppler/GPS navigation? no
Sensors & Samplers
410/120kHz Sidescan sonar850 kHz Sidescan sonor
CTD
Dissolved oxygen
Large capacity slurp samplers (Multi-chamber)
Multibeam maps
Sub bottom profiler
What type of samples do you expect to collect?
if possible, some surficial sediment and water samples from outlying areas in Guaymas Basin that are not visited by Alvin on this cruise. However, additional payload should not interfere with SENTRY's principal use for seafloor photomosaics and shallow subbottom surveys on this cruise. It will be good to discuss some of the sensors and payload with SENTRY experts at the pre-cruise meeting.
Science Supplied Equipment
Are you supplying equipment to be used with AUV Sentry? no
Has this equipment been used on Sentry before? no
Please give a brief description of the equipment, its intended purpose, the cruise # it was last used on if any and its deployment method.
Does this equipment use an external pressure housing? no
Has the pressure case been tested to the anticipated depth of deployment? no
Air weight(s) of this equipment?
Water weight(s) of this equipment?
Does the equipment require data or a power interface from the vehicle?
no
Does the equipment meet the electrical requirements of the Sentry User Manual?
no
Additional Information
Brief operations description or comments: Dan Hoer from Harvard (Pete Girguis) is planning to bring an in-situ mass spectrometer for sniffing out alkanes in Guaymas Basin; however, as far as I remember, this unit will be used on Alvin, not on Sentry.Dirk de Beer (Max-Planck-Institute Bremen) will bring an in-situ microprofiler for measuring chemical and T gradients in surficial sediments on a micrometer scale, to be used with Alvin. The profiler was successfully deployed back in 2009. Reference:doi: 10.3389/fmicb.2016.000075.
Date Submitted: Aug 23, 2016 9:43 PM