Curriculum Vitae

Karen L. Casciotti
Assistant Scientist
Department of Marine Chemistry and Geochemistry
Woods Hole Oceanographic Institution
McLean Lab, MS 8
Woods Hole, MA 02543
phone: (508) 289-3738
fax: (508) 457-2013
email: kcasciotti@whoi.edu

Education
2002 Ph.D., Princeton University; Geosciences.
1999 M.A., Princeton University; Geosciences.
1998 M.S., Scripps Institution of Oceanography, UC San Diego; Oceanography.
1995 B.S., California Institute of Technology; Environmental Engineering Science.

Research Interests
Marine nitrogen cycle, genetics and physiology of nitrifying and denitrifying bacteria, biological control of trace gas production, biology of isotope fractionation, mechanistic modeling of biological isotope fractionation, evolution of biogeochemical cycles.

Research Experience

  • 2004-present Woods Hole Oceanographic Institution.
    Assistant Scientist in the Department of Marine Chemistry and Geochemistry.
  • 2002- 2004 U.S. Geological Survey, NRC Postdoctoral Research Associate.
    Linking molecular and stable isotopic tools to study nitrate dynamics and nitrous oxide production in freshwater and estuarine systems. Dr. Mary Voytek and Dr. J.K. Bohlke, advisors.
  • 1998-2002 Princeton University.
    Molecular and stable isotopic characterization of enzymes involved in nitrification and nitrifier-denitrification. Dr. Bess Ward, advisor.
  • 1997-1998 Scripps Institution of Oceanography, UCSD.
    Stable isotopic composition of atmospheric and soil-derived nitrous oxide. Dr. Martin Wahlen, advisor.
  • 1995-1997 Scripps Institution of Oceanography, UCSD.
    Ultrastructural characterization and localization of manganese oxidizing activity in spores of the marine Bacillus sp. strain SG-1. Dr. Bradley Tebo, advisor.
  • 1995 (summer) University of Hawaii, Manoa.
    Dissolved N2O measurements at Hawaii Ocean Time Series station ALOHA. Dr. David Karl, advisor.
  • 1994 (summer) University of Hawaii, Manoa REU Summer Research Fellowship.
    Development of a new method for the measurement of dissolved DNA and RNA concentrations in sea water. Dr. David Karl, advisor.
  • 1994 California Institute of Technology.
    Measurement of remnant paleomagnetism in rock cores collected from Neoproterozoic glacial deposits in Namibia. Dr. Joe Kirschvink, Dr. David Evans, advisors.
  • 1993 (summer) Center for Great Lakes Research, Milwaukee, WI.
    Characterization of newly isolated bioluminescent bacteria using luxA gene hybridization. Dr. Kenneth Nealson, advisor.
  • 1992-1995 California Institute of Technology.
    Screening mutants in methanotrophy and methylotrophy. Development of fluorescent probes for detection of methanotrophs in the environment. Dr. Mary Lidstrom, advisor.

Publications

  • Coplen, T.B., J.K. Bohlke, and K.L. Casciotti. 2004. Using dual bacterial denitrification to improve 15N determinations of nitrates containing mass independent 17O. Rapid Communications in Mass Spectrometry, 18: 245-250.
  • Casciotti, K.L., D.M. Sigman, and B.B. Ward. 2003. Linking Diversity and Stable Isotope Fractionation in Ammonia-Oxidizing Bacteria. Geomicrobiology Journal, 20(4): 335-353.
  • Casciotti, K.L., D.M. Sigman, M. Galanter Hastings, J.K. Böhlke, and A. Hilkert. 2002. Measurement of the Oxygen Isotopic Composition of Nitrate in Marine and Fresh Waters Using the Denitrifier Method. Analytical Chemistry, 74: 4905-4912.
  • Francis, C.A., K.L. Casciotti, and B.M. Tebo. 2002. Localization of Mn(II)-oxidizing activity and the putative multicopper oxidase, MnxG, to the exosporium of the marine Bacillus sp. strain SG-1. Archives of Microbiology 178: 450-456.
  • Casciotti, K.L. and B.B. Ward. 2001. Dissimilatory Nitrite Reductase Genes in Autotrophic Ammonia-Oxidizing Bacteria. Applied and Environmental Microbiology, 67: 2213-2221.
  • Sigman, D.M., K.L. Casciotti, M. Andreani, C. Barford, M. Galanter, J.K. Böhlke. 2001. A Bacterial Method for the Nitrogen Isotopic Analysis of Nitrate in Marine and Fresh Waters. Analytical Chemistry, 73: 4145-4153.
  • Sigman, D.M., and K.L. Casciotti. 2001. Nitrogen Isotopes in the Ocean. In: Steele, J. H., Thorpe, S. A., and Turekian, K. K. (eds) Encyclopedia of Ocean Sciences pp. 1884-1894. Academic Press, New York.
  • Landry, M.R., S.L. Brown, K.E. Selph, M.R. Abbott, R.M. Letelier, S. Christensen, R.R. Bidigare, and K.L. Casciotti. 2001. Initiation of the Spring Phytoplankton Increase in the Antarctic Polar Front Zone at 170º W. Journal of Geophysical Research-Oceans, 106 (C7): 13903-13915.

Meeting Presentations

  • Casciotti, K. L., M.A. Voytek, and J.K. Bohlke. 2003. Fall AGU Meeting, San Francisco, CA. Oral presentation, "Nitrogen Isotopic Fractionation by In-Stream Populations of Ammonia-Oxidizing Bacteria."
  • Casciotti, K. L., D. M. Sigman, and B. B. Ward. 2002. Fall AGU Meeting, San Francisco, CA. Invited oral presentation, "Linking Diversity and Stable Isotope Fractionation in Ammonia-Oxidizing Bacteria."
  • Casciotti, K. L., D. M. Sigman, and B. B. Ward. 2002. Annual Meeting of the American Society of Limnology and Oceanography, Honolulu, HI. Poster presentation, "Isotope Signatures for N2O Production by Marine Ammonia-Oxidizing Bacteria."
  • Casciotti, K. L., D. M. Sigman, and B. B. Ward. 2001 Gordon Research Conference in Chemical Oceanography, Tilton, NH. Poster presentation, "Isotope Effects for Ammonia Oxidation and Nitrite Reduction by Ammonia-Oxidizing Bacteria."
  • Casciotti, K. L., D. M. Sigman, and B. B. Ward. 2001 Annual Meeting of the American Society of Limnology and Oceanography, Albuquerque, NM. Oral presentation "Molecular and Biogeochemical Similarity of Nitrite Reductase in Nitrifying and Denitrifying Bacteria."
  • Casciotti, K. L., and B. B. Ward. 2000 General Meeting of the American Society for Microbiology, Los Angeles, CA. Poster presentation "Molecular Characterization of Copper-Containing Nitrite Reductases from Ammonia-Oxidizing Bacteria."
  • Casciotti, K. L., and B. B. Ward. 1999 Summer Meeting of the Center for Environmental Bioinorganic Chemistry, Princeton, NJ. Oral presentation "Dissimilatory Nitrite Reductase Gene from Nitrosomonas marina."
  • Casciotti, K. L., T. Rahn, and M. Wahlen. 1997 Fall Meeting of the American Geophysical Union, San Francisco, CA. Poster Presentation "Stable Isotopes of N and O Nitrous Oxide Emissions from Fertilized Soils."
  • Francis, C. A., K. L. Casciotti, and B. M. Tebo. 1997 General Meeting of the American Society for Microbiology, Miami Beach, FL. Poster presentation "Manganese Oxidizing Activity Localized to the Exosporium of Spores of the Marine Bacillus sp. strain SG-1."

Honors and Awards
2002 NRC Postdoctoral Research Associateship
2002 ASLO Annual Meeting Outstanding Student Poster Award
2001 Princeton University Harold W. Dodds Honorific Fellowship
1997 AGU Fall Meeting Outstanding Student Paper-Atmospheric Sciences
1994 NSF Research Experience for Undergraduates (REU) Fellowship


Teaching Assistantships
2000 Princeton University: Oceanography (GEO 202)
1995-1998 University of California, San Diego: Environmental Microbiology (BIMM 126), Bacteriology (BIMM 120), Microbial Genetics (BIMM 122), Zooplankton Ecology (SIO 271)

Field Research Participation
Nov 09-Dec 14, 2000 Denitrification in Lake Bonney, Antarctica
Jun 12-16, 1998 R/V Robert Gordon Sproul, Chief scientist, Southern California Bight
Oct 20-Nov 24, 1997 R/V Roger Revelle, U.S. JGOFS AESOPS Survey I Cruise, Antarctic Polar Front, 170º W
Aug 27-Sep 01, 1995 R/V Moana Wave, Hawaii Ocean Time Series Cruise 65
Jul 28-Aug 02, 1995 R/V Maurice Ewing, Hawaii Ocean Time Series Cruise 64
Aug 20-Sept 17, 1994 Field assistant for paleomagnetic sample collection in Namibia
Jul 23-Jul 28, 1994 R/V Moana Wave, Hawaii Ocean Time Series Cruise 55
Jun 17-Jun 22, 1994 R/V Moana Wave, Hawaii Ocean Time Series Cruise 54


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