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Copyright 1998 Stuart News Company

The Stuart News/Port St. Lucie News (Stuart,FL)

May 10, 1998, Sunday

SECTION: A Section; Pg. A2

LENGTH: 705 words

HEADLINE: FOCUS OF FISH KILL RESEARCH QUESTIONED

BYLINE: Debi Pelletier of the News staff

BODY:

Troubled Waters

After all of this, could it be Critter B?

As Florida researchers prepare to test the St. Lucie Estuary and other state waters for signs of a toxic microorganism they tentatively have linked to fish kills, a leading scientist is sounding a cautionary note.

Cryptoperidiniopsis, the primary suspect in the sick fish mystery, might not be the culprit, despite the state Department of Environmental Protection's focus.

"I keep hearing that the Florida DEP is focusing on Crypto as the likely cause of the fish disease events," said JoAnn Burkholder, a botanist at North Carolina State University's Biohazard 3 laboratory in Raleigh. "I'm really troubled by all this because there's no evidence Crypto is toxic."

Burkholder discovered the toxic form of Pfiesteria piscicida - microalgae closely related to Crypto and blamed for fish kills in the Chesapeake Bay last summer. After waiting for more than two months, she only recently received water samples containing Crypto.

"I think you've probably got several things that could be very much like Pfiesteria," Burkholder said. "It's just that I don't know if Cryptoperidiniopsis is the one to focus on. It could be, but we have no idea. We have to make sure it's toxic first."

Things appear to be winding down in the fish death crisis. Activity at a DEP office charged with taking calls and fish samples in Jensen Beach has slowed almost to a standstill, and the Army Corps of Engineers has decreased freshwater releases from overfull Lake Okeechobee into the St. Lucie River. The releases from the lake and resulting changes in estuary salinity were widely suspected of contributing to the fish kills.

However, scientists plan to continue testing area waters. They hope to compile as much information as they can before a May 22 summit with local, state and federal officials in Stuart.

After seeing the havoc wreaked by Pfiesteria in Chesapeake Bay, state officials created the Florida Harmful Algal Bloom Task Force. The DEP's Karen Steidinger heads the technical advisory committee, which is surveying state waters for potentially toxic microalgae. The only one the researchers have found, other than the more common red tide, is Cryptoperidniopsis.

Because of its similarity to Pfiesteria and its presence where there are sick fish, Crypto has become the chief suspect. However, Burkholder is concerned that by focusing on Crypto, other organisms might be missed.

For example, a microalga she calls Critter B.

It's toxic, it kills fish and it's not Crypto or Pfiesteria, she said.

Burkholder said the organism was discovered in 1993 when her lab received water samples five years ago from Pensacola and the St. Johns and St. Lucie rivers.

"We found Pfiesteria-like species that came up and killed fish," she said.

The microalgae was identified as Pfiesteria, but Burkholder said those criteria were changed.

Steidinger has said no Pfiesteria has been found in Florida so far, according to the current criteria. Burkholder wants to work more closely with her in obtaining samples "to try and determine the toxicity before anybody focuses on any one in particular."

She also questions the value of a statewide survey of microorganisms, unless it's done where there are sick fish.

"In Maryland and in North Carolina, Virginia and Delaware, I've advised people not to do any kind of routine monitoring to try to track something like Pfiesteria, because if you go out and you find areas where Pfiesteria is, it doesn't tell you anything," Burkholder said. "We have areas in North Carolina where there are 5,000 Pfiesteria cells per milliliter and the fish are fine."

She said the key is whether there is toxic activity, so samples must be taken where there are sick or dying fish.

David Heil is the facilitator for the Harmful Algal Bloom Task Force in Tallahassee. He said two papers are being prepared - one to identify the most problematic species of microalgae and another to set research priorities for agencies concerned about fisheries, the economy and public health.

But the task force wants to go one step further, he said, "to see if we can do more than respond, but predict, mitigate and control outbreaks."

LOAD-DATE: May 12, 1998