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Copyright 1998 Palm Beach Newspaper, Inc.

The Palm Beach Post

April 8, 1998, Wednesday, MARTIN-ST. LUCIE EDITION

SECTION: LOCAL, Pg. 1B

LENGTH: 614 words

HEADLINE: BIOLOGIST PROBES FOR KEYS TO SICK FISH

BYLINE: Sally D. Swartz, Palm Beach Post Staff Writer

DATELINE: ST. PETERSBURG

BODY:

Biologist Ann Forstchen put on rubber gloves before she touched the dead mullet.

''I'm not afraid of catching anything,'' the fish-health expert said. ''I just don't want my hands to stink forever.''

With tools that looked like tweezers and nail scissors, she removed dots of tissue from the mullet's skin, gills, kidney and an open sore beside its tail.

Forstchen and four other scientists are trying to find out why 25 species of sick fish have turned up from North Palm Beach to New Smyrna Beach since March 2 - the majority in the St. Lucie and Indian rivers near Stuart.

Dead fish from the Treasure Coast, iced in coolers, arrive daily at the Department of Environmental Protection's Florida Marine Research Institute here.

The institute at the downtown St. Petersburg campus of the University of South Florida has state-of-the-art equipment including a scanning electron microscope that magnifies microbes up to 100,000 times.

It is a microscopic algae called Cryptoperidiniopsis that may be causing the fish on the Treasure Coast to develop lesions and sores. Forstchen hopes to find out where it comes from, how it behaves and what it does to fish.

But the investigation is just beginning, and Forstchen admits scientists may never know all the answers.

''By the end of the month, we hope to have some sort of preliminary diagnosis, or at least to eliminate some theories,'' Forstchen said. ''We may also end up with a lot more questions.''

She said scientists have made progress, noting different species display different symptoms.

Mullet, for example, seem to be the most affected, with symptoms including open sores, bloody lesions and deep ulcers that reveal organs inside.

Despite reports of tumors, none have been found, Forstchen said. She speculated whitish lesioned areas look like tumors when viewed under water.

Jacks have surface lesions rather than gaping holes, she said, and other species have reddened areas that look like surface hemorrhaging or red dots.

''We can't jump to conclusions and say they're all caused by the same thing,'' Forstchen said.

Biologists are growing cultures from tissues of the sick fish to see what bacteria is present. They're treating tissues with special materials to create slides to study, a three-week process.

''We're looking for something different in the fish that we wouldn't >find in the water,'' Forstchen said. ''So far, what's in the fish is in the water.''

Scientists are looking at the sick fish as a problem of chronic fish >disease, rather than an extensive fish kill.

And biologists have not yet found Pfiesteria, the toxic microbe >implicated in fish kills in other states, in Florida. Pfiesteria caused humans to >develop sores and experience temporary memory loss and learning problems.

While Crypto, as its known, looks a lot like Pfiesteria under a >microscope, scientists know of no human health problems associated with it or the lesioned fish.

Crypto seems to like water with lower saltiness, which is certainly the >case on the Treasure Coast. The influx of fresh water, which water managers >released to lower overflowing Lake Okeechobee, could have triggered a bloom of the >toxic microalgae, Forstchen said. Or nutrients from runoff of fresh water could trigger it.

''We don't know,'' Forstchen said. ''Many scientists are convinced >nutrients are a major factor in these events.''

Callers to the state's fish-kill hot line offer creative answers, Forstchen said.

''We've had people blame the nuclear power plant, the full moon, a World >War II submarine leaking mercury, the Drug Enforcement Administration dumping cocaine,'' she said, ''and even aliens.''

GRAPHIC: PHOTO (2 C & B&W), 1. (C) PAUL J. MILETTE/Staff Photographer, Ann Forstchen takes samples from a mullet, one of 25 species of sick fish found >from North Palm Beach to New Smyrna Beach., 2. (C) 'We're looking for something different in the fish that we wouldn't find in the water.' ANN FORSTCHEN Fish-health expert with Florida's Marine Research Institute, 3. (B&W) Photo by Florida Marine Research Institute, Cryptoperidiniopsis as seen through a scanning electron microscope. The algae is believed to have a role in making fish sick.

LOAD-DATE: April 9, 1998