Hahn Lab

List of Publications

(Last updated 15 January 2004)


Hahn, M.E., Karchner, S.I., Franks, D.G., and Merson, R.R. (2004) Aryl hydrocarbon receptor polymorphisms and dioxin resistance in Atlantic killifish (Fundulus heteroclitus). Pharmacogenetics in press. 

Hahn, M.E., Merson, R.R., and Karchner, S.I. (2004) Xenobiotic Receptors in Fishes:  Structural and Functional Diversity and Evolutionary Insights, in Biochemical and Molecular Biology of Fishes.  Vol. 6 - Environmental Toxicology, Moon, T.W. and Mommsen, T.P., Editor., p. in press.


Hahn, M.E., Jensen, B.A., Kim, E.-Y., Karchner, S.I., Franks, D.G., Lapseritis, J.M., Whalen, K.E., and Carvan, M.J. (2003) Molecular and cellular approaches to understanding the sensitivity of marine mammals to persistent organic pollutants. Organohalogen Compounds 62: 253-256. 

Hahn, M.E., Karchner, S.I., and Franks, D.G. (2003) The Ah receptor and its ligands:  A comparative perspective. Organohalogen Compounds 65: 110-113.

Hahn, M.E. (2003) Chapter 14:  Evolutionary and Physiological Perspectives on Ah Receptor Function and Dioxin Toxicity, in Dioxins and Health, Second Edition, Schecter, A. and Gasiewicz, T.A., Editor., John Wiley & Sons: p. 559-602.

Hart, C.A., Nisbet, I.C.T., Kennedy, S.W., and Hahn, M.E. (2003) Gonadal feminization and halogenated environmental contaminants in common terns (Sterna hirundo):  Evidence that ovotestes in male embryos do not persist to the prefledgling stage. Ecotoxicology  12: 125-140.

Meyer, J.N., Wassenberg, D.M., Karchner, S.I., Hahn, M.E., and DiGiulio, R.T. (2003) Expression and inducibility of aryl hydrocarbon receptor (AHR) pathway genes in wild-caught killifish (Fundulus heteroclitus) with different contaminant exposure histories. Environ. Toxicol. Chem. 22: 2337-2343.

Tittlemier, S.A., Kennedy, S.W., Hahn, M.E., Reddy, C.M., and Norstrom, R.J. (2003) Naturally-produced halogenated dimethyl bipyrroles bind to the Ah receptor and induce cytochrome P4501A and porphyrin accumulation in chicken embryo hepatocytes. Environ. Toxicol. Chem. 22: 1497-1506.

Song, J., Clagett-Dame, M., Peterson, R.E., Hahn, M.E., Westler, W.M., Sicinski, R.R., and DeLuca, H.F. (2002) A Novel Ligand for the Aryl Hydrocarbon Receptor Isolated from Lung. Proc. Natl. Acad. Sci. U.S.A.  99: 14694-14699.

Andreasen, E.A., Hahn, M.E., Heideman, W., Peterson, R.E., and Tanguay, R.L. (2002) The zebrafish (Danio rerio) aryl hydrocarbon receptor type 1 (zfAHR1) is a novel vertebrate receptor. Mol. Pharmacol.  62: 234-249.

Billiard, S.M., Hahn, M.E., Franks, D.G., Peterson, R.E., Bols, N.C., and Hodson, P.V. (2002) Binding of polycyclic aromatic hydrocarbons (PAHs) to teleost aryl hydrocarbon receptors (AHRs). Comparative Biochemistry and Physiology B  133: 55-68.

Hahn, M.E. (2002) Aryl hydrocarbon receptors: Diversity and Evolution. Chem.-Biol. Interact.  141: 131-160.

Kim, E.-Y., Hahn, M.E., Iwata, H., Tanabe, S., and Miyazaki, N. (2002) cDNA cloning of an aryl hydrocarbon receptor from Baikal Seals (Phoca sibirica). Mar. Environ. Res.  54: 285-289.

Karchner, S.I., Franks, D.G., Powell, W.H., and Hahn, M.E. (2002) Regulatory Interactions Among Three Members of the Vertebrate Aryl Hydrocarbon Receptor Family: AHR Repressor, AHR1, and AHR2. J. Biol. Chem. 277: 6949-6959.

Powell, W.H. and Hahn, M.E. (2002) Identification and Functional Characterization of Hypoxia-inducible factor 2a from the marine teleost, Fundulus heteroclitus:  Interaction of HIF-2a with two ARNT2 splice variants. J. Exp. Zool.-Molecular and Developmental Evolution  294: 17-29.

Hahn, M.E. (2002) Biomarkers and Bioassays for Detecting Dioxin-like Compounds in the Marine Environment. Science of the Total Environment 289:49-69

Kim, E.-Y. and Hahn, M.E. (2002) cDNA cloning and characterization of an aryl hydrocarbon receptor from the harbor seal (Phoca vitulina):  A biomarker of dioxin susceptibility? Aquat. Toxicol. 58: 57-73

Hestermann, E.V., Stegeman, J.J., and Hahn, M.E. (2002) Serum Withdrawal Leads to Reduced Aryl Hydrocarbon Receptor Expression and Loss of Cytochrome P4501A Inducibility in PLHC-1 Cells. Biochem. Pharmacol. 63: 1405-1414

Hestermann, E.V., Stegeman, J.J., and Hahn, M.E. (2002) Relationships Among the Cell Cycle, Cell Proliferation, and Aryl Hydrocarbon Receptor Expression in PLHC-1 Cells. Aquat. Toxicol.58: 201-213


Butler, R.B., Kelley, M.L., Powell, W.H., Hahn, M.E., and Van Beneden, R.J. (2001) An Aryl Hydrocarbon Receptor Homologue from the Soft-Shell Clam, Mya arenaria:  Evidence that invertebrate AHR homologues lack TCDD and BNF binding.   Gene278: 223-234.

Jensen, B.A. and Hahn, M.E. (2001) cDNA cloning and characterization of a high affinity aryl hydrocarbon receptor in a cetacean, the beluga, Delphinapterus leucas. Toxicol. Sci.  64: 41-56.

Bello, S.M., Franks, D.G., Stegeman, J.J., and Hahn, M.E. (2001) Acquired Resistance to Aryl Hydrocarbon Receptor Agonists in a Population of Fundulus heteroclitus from a Marine Superfund site:  In Vivo and In Vitro Studies on the Induction of Xenobiotic Metabolizing Enzymes. Toxicol. Sci.  60: 77-91.

Hahn, M.E. (2001) Dioxin Toxicology and the Aryl Hydrocarbon Receptor:  Insights from fish and other non-traditional models. Marine Biotechnology  3(Suppl.1): S224-S238.

Oleksiak, M.F., Wu, S., Parker, C., Karchner, S.I., Stegeman, J.J., and Zeldin, D.C. (2000) Identification, functional characterization and regulation of a new cytochrome P450 subfamily, the CYP2Ns. J. Biol. Chem.  275: 2312-2321.

Hahn, M.E. and Stegeman, J.J. (2000) Opportunities for Environmental Applications of Marine Biotechnology.  (Proceedings of the Workshop on Opportunities for Advancement of Environmental Applications of Marine Biotechnology.  October 5-6, 1999, Washington, DC), Washington, DC, National Academy Press: pp. 112-125.

Hestermann, E.V., Stegeman, J.J., and Hahn, M.E. (2000) Relative contributions of affinity and intrinsic efficacy to aryl hydrocarbon receptor ligand potency. Toxicol. Appl. Pharmacol.  168(2): 160-172.

Powell, W.H., Bright, R., Bello, S.M., and Hahn, M.E. (2000) Developmental and Tissue-specific Expression of AHR1, AHR2, and ARNT2 in Dioxin-sensitive and -resistant Populations of the Marine Fish, Fundulus heteroclitus. Toxicol. Sci.  57: 229-239.

Quadri, S.A., Qadri, A.N., Hahn, M.E., Mann, K.K., and Sherr, D.H. (2000) The Bioflavonoid Galangin Blocks Aryl Hydrocarbon Receptor (AhR) Activation and Polycyclic Aromatic Hydrocarbon-induced Pre-B cell Apoptosis. Mol. Pharmacol.  58: 515-525.

Toomey, B.H., Bello, S., Hahn, M.E., Cantrell, S., Wright, P., Tillitt, D., and DiGiulio, R.T. (2001) TCDD induces apoptotic cell death and cytochrome P4501A expression in developing Fundulus heteroclitus embryos. Aquat. Toxicol.  53: 127-138.

White, R.D., Shea, D., Schlezinger, J.J., Hahn, M.E., and Stegeman, J.J. (2000) In vitro metabolism of polychlorinated biphenyl congeners by beluga whale (Delphinapterus leucas) and pilot whale (Globicephala melas) and relationship to cytochrome P450 expression. Comp. Biochem. Physiol.  126: 267-284.

Karchner, S.I., Kennedy, S.W., Trudeau, S., and Hahn, M.E. (2000) Towards a Molecular Understanding of Species Differences in Dioxin Sensitivity:  Initial Characterization of Ah Receptor cDNAs in Birds and an Amphibian. Mar. Environ. Res.  50: 51-56.

Powell, W.H. and Hahn, M.E. (2000) The evolution of aryl hydrocarbon signaling proteins: Diversity of ARNT isoforms among fish species. Mar. Environ. Res. 50: 39-44.

Hestermann, E.V., Stegeman, J.J., and Hahn, M.E. (2000) Serum alters the uptake and relative potencies of halogenated aromatic hydrocarbons in cell culture bioassays. Toxicol. Sci.  53: 316-325.

Huuskonen, S.E., Tuvikene, A., Trapido, M., Fent, K., and Hahn, M.E. (2000) CYP1A induction and porphyrin accumulation in PLHC-1 fish cells exposed to sediment and oil shale extracts. Arch. Environ. Contam. Toxicol.  38: 59-69.


Karchner, S.I., Powell, W.H., and Hahn, M.E. (1999) Structural and Functional Characterization of Two Highly Divergent Aryl Hydrocarbon Receptors in the teleost Fundulus heteroclitus.  Evidence for a novel class of ligand-binding bHLH-PAS factors. Journal of Biological Chemistry  274: 33814-33824 .

Abnet, C.C., Tanguay, R.L., Hahn, M.E., Heideman, W., and Peterson, R.E. (1999) Two forms of aryl hydrocarbon receptor type 2 in rainbow trout (Oncorhynchus mykiss):  Evidence for differential expression and enhancer specificity. Journal of Biological Chemistry  274: 15159-15166.

Mann, K.K., Matulka, R.A., Hahn, M.E., Trombino, A.F., Lawrence, B.P., Kerkvliet, N.I., and Sherr, D.H. (1999) The Role of polycyclic aromatic hydrocarbon metabolism in dimethylbenzanthracene-induced Pre-B Cell Apoptosis. Toxicol. Appl. Pharmacol.  161: 10-22.

Powell, W.H., Karchner, S.I., Bright, R., and Hahn, M.E. (1999) Functional diversity of vertebrate ARNT proteins:  Identification of ARNT2 as the predominant form of ARNT in the marine teleost, Fundulus heteroclitus. Archives of Biochemistry and Biophysics 361: 156-163.

Hahn, M.E. (1998) Mechanisms of innate and acquired resistance to dioxin-like compounds. Reviews in Toxicology.  Series B - Environmental Toxicology  2: 395-443.

Hahn, M.E. (1998) The Aryl Hydrocarbon Receptor: A Comparative Perspective. Comp. Biochem. Physiol. 121C(3):23-53.

Hahn, M.E., Woodin, B.R., Stegeman, J.J., and Tillitt, D.E. (1998) Aryl hydrocarbon receptor function in early vertebrates: Inducibility of cytochrome P4501A in agnathan and elasmobranch fish. Comp. Biochem. Physiol. 120C: 67-75.

Hahn, M.E., Karchner, S.I., Shapiro, M.A., and Perera, S.A. (1998) The aryl hydrocarbon receptor in early vertebrates. Mar. Environ. Res.  46: 41-44.

Morrison, H.G., Weil, E.J., Karchner, S.I., Sogin, M.L., and Stegeman, J.J. (1998) Molecular cloning of CYP1A from the estuarine fish Fundulus heteroclitus and phylogenetic analysis of CYP1A genes: Update with new sequences. Comp. Biochem. Physiol.  121C: 231-240.

Besselink, H.T., Denison, M.S., Hahn, M.E., Karchner, S.I., Vethaak, A.D., Koeman, J.H., and Brouwer, A. (1998) Low inducibility of CYP1A activity by polychlorinated biphenyls (PCBs) in flounder (Platichthys flesus): Characterization of the Ah receptor and the role of CYP1A inhibition. Toxicol. Sci.  43: 161-171.

Fragoso, N.M., Parrott, J.L., Hahn, M.E., and Hodson, P.V. (1998) Chronic retene exposure causes sustained induction of CYP1A activity and protein in rainbow trout (Oncorhynchus mykiss). Environ. Toxicol. Chem.  17(11): 2347-2354.

Huuskonen, S.E., Ristola, T.E., Tuvikene, A., Hahn, M.E., Kukkonen, J.V.K., and Lindstrom-Seppa, P.E. (1998) Comparison of two bioassays, a fish liver cell line (PLHC-1) and a midge (Chironomus riparius), in monitoring freshwater sediments. Aquatic Toxicology  44: 47-67..

Huuskonen, S.E., Hahn, M.E., and Lindstrom-Seppa, P.E. (1998) A fish hepatoma cell line (PLHC-1) as a tool to study cytotoxicity and CYP1A induction properties of cellulose and wood chip extracts. Chemosphere  36: 2921-2932.

Hahn, M.E., Karchner, S.I., Shapiro, M.A., and Perera, S.A. (1997) Molecular evolution of two vertebrate aryl hydrocarbon (dioxin) receptors (AHR1 and AHR2) and the PAS family. Proc. Natl. Acad. Sci. U.S.A. 94: 13743-13748

M. Celander, J. Bremer, M.E. Hahn, and J.J. Stegeman (1997) Glucocorticoid-xenobiotic interactions: Dexamethasone potentiation of cytochrome P4501A induction by b-naphthoflavone in a fish hepatoma cell line (PLHC-1). Environmental Toxicology and Chemistry 16: 900-907.

A. Lorenzen, S.W. Kennedy, L.J. Bastien, and M.E. Hahn (1997) Halogenated aromatic hydrocarbon-mediated porphyrin accumulation and induction of P4501A in chicken embryo hepatocytes. Biochemical Pharmacology 53: 373-384.


S.W. Kennedy, A. Lorenzen, S.P. Jones, M.E. Hahn, and J.J. Stegeman (1996) Cytochrome P4501A induction in avian hepatocyte cultures: a promising approach for predicting the sensitivity of avian species to in ovo toxic effects of halogenated aromatic hydrocarbons. Toxicology and Applied Pharmacology 141: 214-230.

M.E. Hahn and K. Chandran (1996) Uroporphyrin accumulation associated with cytochrome P4501A induction in fish hepatoma cells exposed to aryl hydrocarbon receptor agonists, including 2,3,7,8-tetrachlorodibenzo-p-dioxin and planar chlorobiphenyls. Archives of Biochemistry and Biophysics 329(2): 163-174.

M. Celander, M.E. Hahn, and J.J. Stegeman (1996) Cytochromes P450 (CYP) in the Poeciliopsis lucidahepatocellular carcinoma cell line (PLHC-1): Dose- and time-dependent glucocorticoid potentiation of CYP1A induction without induction of CYP3A. Archives of Biochemistry and Biophysics 329(1): 113-122.

M.E. Hahn (1996) Ah receptors and the mechanism of dioxin toxicity: Insights from homology and phylogeny, in Interconnections Between Human and Ecosystem Health, DiGiulio, R.D. and Monosson, E., (Eds.), Chapman and Hall Publishers (pp. 9-26).

M.E. Hahn, B.L. Woodward, J.J. Stegeman, and S.W. Kennedy (1996) Rapid assessment of induced cytochrome P4501A (CYP1A) protein and catalytic activity in fish hepatoma cells grown in multi-well plates. Environmental Toxicology and Chemistry 15(4): 582-591.

S.I. Karchner and M.E. Hahn (1996) A reverse transcription-polymerase chain reaction (RT-PCR) approach for cloning Ah receptors from diverse vertebrate species: partial sequence of an Ah receptor from the teleost Fundulus heteroclitus. Marine Environmental Research 42(1-4): 13-17.

M.E. Hahn and S.I. Karchner (1995) Evolutionary conservation of the vertebrate Ah (dioxin) receptor: Amplification and sequencing of the PAS domain of a teleost Ah receptor cDNA. Biochemical Journal 310: 383-387. killifish AHR2 sequence (GenBank Accession Number U29679)

N.W. Cornell, M.E. Hahn, and H.A. Martin (1995) Characterization and use of isolated toadfish hepatocytes for studies of heme synthesis and utilization. Biological Bulletin 189: 227-228 (short report).

J.J. Stegeman, M.E. Hahn, R. Weisbrod, B.R. Woodin, J.S. Joy, S. Najibi, and R.A. Cohen (1995) Induction of cytochrome P4501A1 by Ah-receptor agonists in porcine aorta endothelial cells in culture, and CYP1A1 activity in intact cells. Molecular Pharmacology 47: 296-306.


R.D. White, M.E. Hahn, W.L. Lockhart, and J.J. Stegeman (1994) The hepatic microsomal cytochrome P450 system in beluga whales (Delphinapterus leucas). Toxicology and Applied Pharmacology 126: 45-57

P. Lindstrom-Seppa, P.J. Korytko, M.E. Hahn, and J.J. Stegeman (1994) Uptake of waterborne 3,3',4,4'-tetrachlorobiphenyl and organ and cell-specific induction of cytochrome P4501A in the fathead minnow Pimephales promelas. Aquatic Toxicology 28: 147-167

M.E. Hahn (1994) Using Biomarkers to Detect Contamination of the Marine Environment. Nor'easter (Magazine of the Northeast Sea Grant Programs) 6: 8-11

M.E. Hahn (1994) Cytochrome P450 induction and inhibition by planar halogenated aromatic hydrocarbons in a fish cell line: promise and pitfalls for environmental testing. In Vitro Cell and Developmental Biology 30A: 39-40 (Abstract T-8)

M.E. Hahn, A. Poland, E. Glover, and J.J. Stegeman (1994) Photoaffinity labeling of the Ah receptor: phylogenetic survey of diverse vertebrate and invertebrate species. Archives of Biochemistry and Biophysics 310: 218-228

M.E. Hahn and J.J. Stegeman (1994) Regulation of cytochrome P4501A1 in teleosts: Sustained induction of CYP1A1 mRNA, protein, and catalytic activity by 2,3,7,8-tetrachlorodibenzofuran in the marine fish Stenotomus chrysops. Toxicology and Applied Pharmacology 127: 187-198

Stegeman, J.J. and M.E. Hahn (1993) Biochemistry and molecular biology of monooxygenases: Current directions in forms, functions, and regulation of cytochrome P450 in aquatic species, in Aquatic Toxicology: Molecular, Biochemical and Cellular Perspectives, Malins, D.C. and Ostrander, G.K., Editors, CRC/Lewis, pp. 87-206

M.E. Hahn, T.M. Lamb, M.E. Schultz, R.M. Smolowitz, and J.J. Stegeman (1993) Cytochrome P4501A induction and inhibition by 3,3',4,4'-tetrachlorobiphenyl in an Ah receptor-containing fish hepatoma cell line (PLHC-1). Aquatic Toxicology 26: 185-208.


M.E. Hahn, A. Poland, E. Glover, and J.J. Stegeman (1992) The Ah receptor in marine animals: Phylogenetic distribution and relationship to cytochrome P4501A inducibility. Marine Environmental Research 34: 87-92

M.E. Hahn and J.J. Stegeman (1992) Phylogenetic distribution of the Ah receptor in non-mammalian species: implications for dioxin toxicity and Ah receptor evolution. Chemosphere 25: 931-937

R.M. Smolowitz, M.E. Hahn, and J. J. Stegeman (1991) Immunohistochemical localization of cytochrome P450IA1 induced by 3,3',4,4'-tetrachlorobiphenyl and by 2,3,7,8-tetrachlorodibenzofuran in liver and extrahepatic tissues of the teleost Stenotomus chrysops (scup). Drug Metabolism and Disposition 19: 113-123

M.E. Hahn and T.A. Gasiewicz (1991) Determination of individual porphyrins using high-performance liquid chromatography following cleanup by anion exhange chromatography. Journal of Chromatography 563: 363-368

J. J. Stegeman, R.M. Smolowitz, and M.E. Hahn (1991) Immunohistochemical localization of environmentally induced cytochrome P450IA1 in multiple organs of the marine teleost Stenotomus chrysops (scup). Toxicology and Applied Pharmacology 110: 486-504

M.E. Hahn and J.J. Stegeman (1990) The role of biotransformation in the toxicity of marine pollutants. In: Pour L'Avenir du Beluga (For the Future of the Beluga, Proceedings of the International Forum for the Future of the Beluga, Tadoussac, Quebec, Canada, 29 September - 1 October, 1988) (J. Prescott & M. Gauquelin, Eds.) pp. 185-198; Presses de l'Universite du Quebec, Sillery, Quebec.

M.E. Hahn, J.A. Goldstein, P. Linko, and T.A. Gasiewicz (1989) Interaction of hexachlorobenzene with the receptor for 2,3,7,8-tetrachlorodibenzo-p-dioxin in vitro and in vivo. Evidence that hexachlorobenzene is a weak Ah receptor agonist. Archives of Biochemistry and Biophysics 270: 344-355

M.E. Hahn, B.R. Woodin, and J.J. Stegeman (1989) Induction of cytochrome P450E (P450IA1) by 2,3,7,8-tetrachlorodibenzofuran (2,3,7,8-TCDF) in the marine fish scup (Stenotomus chrysops). Marine Environmental Research 28: 61-65

J.W. Gooch, A.A. Elskus, P.J. Kloepper-Sams, M.E. Hahn, and J.J. Stegeman (1989) Effects of ortho and non-ortho substituted polychlorinated biphenyl congeners on the hepatic monooxygenase system in scup (Stenotomus chrysops). Toxicology and Applied Pharmacology 98: 422-433

M.E. Hahn, T.A. Gasiewicz, P. Linko, and J.A. Goldstein (1988) The role of the Ah locus in hexachlorobenzene-induced porphyria: studies in congenic C57BL/6J mice. Biochemical Journal 254: 245-254.

Goldstein JA, Linko P, Hahn ME, Gasiewicz TA, Yeowell HN. (1986) Structure-activity relationships of chlorinated benzenes as inducers of hepatic cytochrome P-450 isozymes in the rat. IARC Sci Publ 77: 519-526

For reprints or additional information, contact:

Mark E. Hahn, Ph.D.
Associate Scientist
Biology Department, MS-32
Woods Hole Oceanographic Institution
Woods Hole, MA 02543-1049

email: mhahn@whoi.edu

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