Possible influence of bacterial quorum sensing on the hydrolysis of sinking particulate organic carbon in marine environments

Quorum sensing control of phosphorus acquisition in Trichodesmium consortia.

Composition and fate of gas and oil released to the water column during the Deepwater Horizon spill.

Rapid microbial respiration of Deepwater Horizon oil in surface waters of the Gulf of Mexico

Microbial sources of intact polar diacylglycerolipids in the western North Atlantic Ocean

Phosphorus supply drives rapid turnover of membrane phospholipids in the diatom Thalassiosira pseudonanna.

Bacterial and eukaryotic intact polar lipids in the eastern subtropical South Pacific: water-column distribution, planktonic sources, and fatty acid composition.

Abundance and diversity of heterotrophic bacterial cells assimilating 33P-phosphate in the subtropical North Atlantic Ocean

Tracking hydrocarbon plume transport and biodegradation at Deepwater Horizon

Viral glycosphingolipids induce lytic infection and cell death in marine phytoplankton

Kinetic constraints on acylated homoserine lactone-based quorum sensing in marine environments

Phytoplankton in the ocean substitute lipids in response to phosphorus scarcity

An interlaboratory study of TEX86 and BIT analysis using high-performance liquid chromatography-mass spectrometry

Assessing nutrient limitation of Prochlorococcus in the North Pacific subtropical gyre by using an RNA capture method

Phospholipid synthesis rates in the eastern subtropical South Pacific Ocean

Barium in twilight zone suspended matter as a proxy for particulate organic carbon mineralization: Results for the North Pacific

Primary, new and export production in the NW Pacific Subarctic Gyre during the VERTIGO K2 experiments

Microbial vs. zooplankton control of sinking particle flux in the ocean's twilight zone

Phosphate availability: ultimate control of new nitrogen input by nitrogen fixation in the tropical Pacific Ocean

Growth and P-specific uptake rates of bacterial and phytoplanktonic communities in the Southeast Pacific (BISOSOPE cruise)

Microbes and the marine phosphorus cycle.

Revisiting carbon flux through the ocean's twilight zone.

Sulfolipids dramatically decrease phosphorus demand by picocyanobacteria in oligotrophic marine environments.

Quantifying 3H-thymidine incorporation rates by a phylogenetically defined group of marine planktonic bacteria (Bacteriodetes phylum).

Relationships between bacterial community structure, light, and carbon cycling in the eastern subarctic North Pacific

Impact of suboxia on sinking particulate organic carbon: Enhanced carbon flux and preferential degradation of amino acids via denitrification

Seasonal variation in sedimentary amino acids and the association of organic matter with mineral surfaces in a sandy eelgrass meadow

Evidence of tight coupling between bacteria and particulate organic carbon during seasonal stratification of Lake Michigan.

Microbiological, molecular biological and stable isotope evidence for nitrogen fixation in the open waters of Lake Michigan

General Interest Articles

Assessing nutrient limitation of Prochlorococcus in the North Pacific subtropical gyre by using an RNA capture method

Van Mooy, B.A.S. and A.H. Devol

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» Assessing nutrient limitation of Prochlorococcus in the North Pacific subtropical gyre by using an RNA capture method

Limnol. Oceanogr. 53:78-88. (2008)

Abstract
It has been hypothesized that the planktonic community of the North Pacific subtropical gyre (NPSG) underwent a &quot;domain shift&quot; in the early 1980s in which phytoplankton of the domain Eukarya were supplanted by phytoplankton of the domain Bacteria, primarily Prochlorococcus. P limitation of eukaryotic phytoplankton was implicated as the causative chemical factor in the domain shift, and we sought to investigate the current nutrient limitation status of Prochlorococcus, now 2 decades since this event. We measured ribonucleic acid (RNA) synthesis rates by NPSG plankton at Station ALOHA in super(33)PO sub(4) super(3-) tracer incubations and found that RNA synthesis was the single largest biochemical sink for dissolved P, accounting for about half of the total PO sub(4) super(3-) uptake. We also found that NH sub(4) super(+) stimulated RNA synthesis but that PO sub(4) super(3-) did not, which suggested N limitation of plankton growth. We developed a new RNA capture procedure, termed radioisotope-based tracking of RNA synthesis by hybridization and capture (RIBOTRACE), to measure RNA synthesis rates by Prochlorococcus exclusively. Data from this procedure showed that NH sub(4) super(+) stimulated RNA synthesis by Prochlorococcus and confirmed that Prochlorococcus was N limited and not P limited. Our RIBOTRACE data do not necessarily refute the domain shift hypothesis, but suggest that any critical period of P limitation required for the domain shift must have subsided and given way to the N- limiting conditions that existed previously.

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