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Systems and methods for submersible imaging flow apparatus

Inventors: Heidi Sosik, Robert Olson
Serial #: 11/978,246

Applications: The Imaging Flow Cytobot (IFCB) is an in-situ automated submersible imaging flow cytometer that generates images of organisms and particles from the aquatic environment. Images can be classified to the genus or even species level with demonstrated accuracy comparable to that of human experts.

Technology: The IFCB uses a combination of flow cytometric and video technology to capture high resolution images of suspended organisms and particles. Laser-induced fluorescence and light scattering from individual particles are measured and used to trigger targeted image acquisition; the optical and image data are then transmitted to shore in real time. Collected images during continuous monitoring can be processed externally with automated image classification software. IFCB generates high resolution (1 µm) images of suspended organisms and particles in the size range <10 to 100 μm (such as diatoms and dinoflagellates). The instrument continuously samples at a rate of 15ml of sea water per hour. Depending on the target population, the IFCB can generate on the order of 10,000 high resolution images per hour.

Last updated: January 7, 2015