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Woods Hole Oceanographic Institution

Tim Verslycke

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Publications
»Copepod diapause
»Lobster Shell Disease
»Crustacean molting receptor
»Lobster Shell Disease
»Mysids as test models for endocrine disruption testing
»Chlorotriazines in the Scheldt estuary
»Energy allocation in grasshopper
»Estrogens in Scheldt estuary
»Marsupial development in mysids to evaluate endocrine disruption
»B[a]P effects on steroid metabolism in mysid
»Ciona CYP3 genes
»Methoprene, nonylphenol, and estrone effects on mysid vitellogenesis
»Methoprene effects on mysid molting
»Mysid growth
»Mysid vitellin ELISA
»Mysid vitellin
»An analytical method to detect estrogens in water
»High levels of endocrine disruptors in wild mysid populations
»Energy allocation in wild mysid populations
»Cellular energy allocation validation with scope for growth
»Dolphin delivery prediction
»PhD thesis
»Endocrine disruptor effects on steroid and energy metabolism in mysid
»Mysid review
»TBT effects on steroid metabolism in mysid
»Metal mixture toxicity to mysid
»TBT effects on energy metabolism in mysid
»dichlorobenzene effects in zebrafish
»Ethinylestradiol effects on amphipod sexual development
»Metabolic studies with mysids
»Abiotic stress and energy metabolism in mysid
»Induced vitellogenesis in rainbow trout
»Steroid metabolism in mysid
»Endocrine disruption in freshwater snails
»Invasive mysid in Belgium


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Tim Verslycke, Endocrine Disruption in the estuarine invertebrate Neomysis integer (Crustacea: Mysidacea), 2003

The aim of this doctoral study was to investigate endocrine disruption in an ecologically relevant invertebrate test species, the mysid shrimp Neomysis integer (Crustacea: Mysidacea), through laboratory and field research. More specifically, the steroid and energy metabolism of N. integer were explored as endpoints to evaluate environmental endocrine disruption.
The thesis consists of 12 chapters, starting with an introduction to the environmental endocrine hypothesis and the conceptual framework of the study. Next, a global assessment is given of the state-of-the-science on endocrine disruption in mysid shrimp. In the third chapter, a new methodology (cellular energy allocation, CEA) to assess the energy budget was adopted for N. integer. In chapter 4, the CEA methodology was evaluated following acute exposure to the antifoulant tributyltinchloride (TBTCl). In chapter 5, the responses of N. integer following exposure to environmentally realistic concentrations of the organophosphate pesticide chlorpyrifos were compared using the CEA and scope for growth (SFG) assays. In chapter 6, testosterone metabolism by N. integer was assessed to obtain initial data on the metabolic capacity. In chapter 7, the effects of TBTCl on the phase I and phase II testosterone metabolism of N. integer were evaluated. In chapter 8, a diverse set of reference compounds suspected of having an endocrine-disrupting mode of action were tested for acute toxicity. In addition, the short-term sublethal effects of methoprene and nonylphenol on the energy and steroid metabolism of N. integer were evaluated. In chapter 9, sediment and mysids (N. integer) from the Scheldt estuary, one of the largest and most polluted estuaries in Western Europe, were analyzed for a number of endocrine disruptors. In chapter 10, the seasonal and spatial patterns in cellular energy allocation of N. integer were investigated in the Scheldt estuary over a 2-year period. In chapter 11, the applicability of the testosterone metabolism assay in N. integer was investigated under field conditions in the Scheldt estuary. In chapter 12, general conclusions and future perspectives of this doctoral study were formulated.
This doctoral study demonstrated that hormone-regulated processes in N. integer can be disrupted by acute exposure to chemicals at environmentally relevant concentrations. http://hdl.handle.net/1854/295

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