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Woods Hole Oceanographic Institution

Tim Verslycke

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Publications
»Copepod diapause
»Lobster Shell Disease
»Crustacean molting receptor
»Lobster Shell Disease
»Mysids as test models for endocrine disruption testing
»Chlorotriazines in the Scheldt estuary
»Energy allocation in grasshopper
»Estrogens in Scheldt estuary
»Marsupial development in mysids to evaluate endocrine disruption
»B[a]P effects on steroid metabolism in mysid
»Ciona CYP3 genes
»Methoprene, nonylphenol, and estrone effects on mysid vitellogenesis
»Methoprene effects on mysid molting
»Mysid growth
»Mysid vitellin ELISA
»Mysid vitellin
»An analytical method to detect estrogens in water
»High levels of endocrine disruptors in wild mysid populations
»Energy allocation in wild mysid populations
»Cellular energy allocation validation with scope for growth
»Dolphin delivery prediction
»PhD thesis
»Endocrine disruptor effects on steroid and energy metabolism in mysid
»Mysid review
»TBT effects on steroid metabolism in mysid
»Metal mixture toxicity to mysid
»TBT effects on energy metabolism in mysid
»dichlorobenzene effects in zebrafish
»Ethinylestradiol effects on amphipod sexual development
»Metabolic studies with mysids
»Abiotic stress and energy metabolism in mysid
»Induced vitellogenesis in rainbow trout
»Steroid metabolism in mysid
»Endocrine disruption in freshwater snails
»Invasive mysid in Belgium


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Tim Verslycke, Stephen D. Roast, John Widdows, Malcolm B. Jones, Colin R. Janssen, Cellular energy allocation and scope for growth in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) following chlorpyrifos exposure: a method comparison, Journal of Experimental Marine Biology and Ecology, 306 (1): 1-16, 2004

Mysids (Crustacea: Mysidacea) are used routinely in acute toxicity testing to evaluate the comparative toxicity of chemicals to aquatic organisms. The need for sublethal endpoints that provide comprehensive understanding of the potential impacts of toxicants to natural populations has resulted in examination of several physiological responses in mysid shrimp, including scope for growth (SFG) and cellular energy allocation (CEA). Both assays, based on the concept that energy in excess of that required for normal maintenance will be available for growth and reproduction, have been reported independently for the mysid Neomysis integer. The present study compares the responses of N. integer following exposure to environmentally realistic concentrations of the organophosphate pesticide chlorpyrifos using both assays. Oxygen consumption in the SFG assay was significantly correlated with cellular respiration rate in the CEA assay, and both were significantly increased by chlorpyrifos exposure. In addition, the protein, sugar, lipid and total energy content in the CEA assay and the egestion rate in the SFG assay were significantly different in chlorpyrifos-exposed mysids compared with control mysids. In contrast, absorption efficiency in the SFG assay was unaffected by pesticide exposure. Significant effects in the SFG and CEA assays were more pronounced following short (i.e. 48 h) compared with longer exposure periods (e.g. 168 h). SFG was significantly reduced at near-lethal concentrations (0.072 and 0.100 µg chlorpyrifos l−1), whereas CEA was reduced in all chlorpyrifos-exposed mysids (0.038, 0.056, 0.072 and 0.100 µg chlorpyrifos l−1) although there was no concentration response. doi:10.1016/j.jembe.2003.12.022




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