Woods Hole Oceanographic Institution

Cruise Planning Synopsis


OC469-1

Ship

R/V Oceanus

Cruise Party

Dennis McGillicuddy: Chief Scientist, Principal Investigator
Woods Hole Oceanographic Institution Bigelow 209A, MS#11 Woods Hole, Ma. USA 02543
+1 508 289 2683
mcgillic@whoi.edu
- added Dennis McGillicuddy as Chief Scientist on Aug 3, 2010 9:58 AM by Eric Benway
- added Dennis McGillicuddy as Principal Investigator on Aug 3, 2010 9:58 AM by Eric Benway

Departure: Oct 1, 2010

Woods Hole

Arrival: Oct 21, 2010

Bridgetown, Barbados

Operations Area

Lat/Lon:
Depth Range: min / max (m)
- set max to max (m) on Aug 3, 2010 9:58 AM by Eric Benway
Will the vessel be operating within 200 NM of a foreign country? yes
- yes on Aug 3, 2010 9:58 AM by Eric Benway
Are visas or special travel documents required? no
- set to no on Aug 3, 2010 9:58 AM by Eric Benway

Science Objectives

The diazotroph Trichodesmium spp. constitutes a major pathway of nitrogen flow into marine planktonic ecosystems, but estimates of its impact on global nitrogen budgets vary widely. Sampling is made difficult by the fragility of the organism with the consequence that Trichodesmium spp. are difficult to manipulate in both field and laboratory experiments. Optical methods that sample the organism nondestructively are thus appealing. A recent transatlantic survey using the Video Plankton Recorder (VPR) revealed unexpectedly high abundance of Trichodesmium spp. at depth, suggesting the vertical distribution of the organism within the euphotic zone may be more uniform than previously thought (Davis, C.S. and McGillicuddy, D.J., 2006. Transatlantic Abundance of the N2-Fixing Colonial Cyanobacterium Trichodesmium. Science, 312: 1517-1520). Application of a simple bio-optical model of productivity to the observed profile of abundance suggests the depth-integrated nitrogen fixation rate could be three to five times higher than that based on the canonical profile of exponential decrease in abundance with depth. However, the observations described in Davis and McGillicuddy (2006) come from a latitude range where Trichodesmium spp. are not especially abundant. This raises a key question: is there a similar vertical distribution in waters further to the south, where Trichodesmium spp. are an order of magnitude more abundant overall? If so, are the deep populations actively fixing nitrogen? If so, the implications for the global nitrogen budget would be substantial.

To answer these questions, we will undertake two cruises to survey the waters of the southern Sargasso Sea and tropical Atlantic, where Trichodesmium spp. are commonly found in high abundance. Along-track VPR measurements will document the abundance and distribution of the organism on the scale of meters to thousands of kilometers. Standard hydrographic station work will provide for comparison of VPR-based estimates with microscope counts, as well as some additional in situ optical methods. A combination of nifH gene expression assays and direct determinations of N2-fixation rates will be made to assess whether or not the deep populations are actively fixing nitrogen. These observations will be synthesized in the context of an eddy-resolving numerical model. This will permit investigation of the mechanisms controlling the vertical and horizontal distribution and abundance of Trichodesmium spp. at multiple scales, including the enigmatic association of relative maxima in abundance with anticyclonic eddies (also described in Davis and McGillicuddy, 2006). Moreover, integration of these observations into the numerical model will facilitate revised estimates of nitrogen fixation by Trichodesmium spp. in the North Atlantic.

-

The diazotroph Trichodesmium spp. constitutes a major pathway of nitrogen flow into marine planktonic ecosystems, but estimates of its impact on global nitrogen budgets vary widely. Sampling is made difficult by the fragility of the organism with the consequence that Trichodesmium spp. are difficult to manipulate in both field and laboratory experiments. Optical methods that sample the organism nondestructively are thus appealing. A recent transatlantic survey using the Video Plankton Recorder (VPR) revealed unexpectedly high abundance of Trichodesmium spp. at depth, suggesting the vertical distribution of the organism within the euphotic zone may be more uniform than previously thought (Davis, C.S. and McGillicuddy, D.J., 2006. Transatlantic Abundance of the N2-Fixing Colonial Cyanobacterium Trichodesmium. Science, 312: 1517-1520). Application of a simple bio-optical model of productivity to the observed profile of abundance suggests the depth-integrated nitrogen fixation rate could be three to five times higher than that based on the canonical profile of exponential decrease in abundance with depth. However, the observations described in Davis and McGillicuddy (2006) come from a latitude range where Trichodesmium spp. are not especially abundant. This raises a key question: is there a similar vertical distribution in waters further to the south, where Trichodesmium spp. are an order of magnitude more abundant overall? If so, are the deep populations actively fixing nitrogen? If so, the implications for the global nitrogen budget would be substantial.

To answer these questions, we will undertake two cruises to survey the waters of the southern Sargasso Sea and tropical Atlantic, where Trichodesmium spp. are commonly found in high abundance. Along-track VPR measurements will document the abundance and distribution of the organism on the scale of meters to thousands of kilometers. Standard hydrographic station work will provide for comparison of VPR-based estimates with microscope counts, as well as some additional in situ optical methods. A combination of nifH gene expression assays and direct determinations of N2-fixation rates will be made to assess whether or not the deep populations are actively fixing nitrogen. These observations will be synthesized in the context of an eddy-resolving numerical model. This will permit investigation of the mechanisms controlling the vertical and horizontal distribution and abundance of Trichodesmium spp. at multiple scales, including the enigmatic association of relative maxima in abundance with anticyclonic eddies (also described in Davis and McGillicuddy, 2006). Moreover, integration of these observations into the numerical model will facilitate revised estimates of nitrogen fixation by Trichodesmium spp. in the North Atlantic.

on Aug 3, 2010 9:58 AM by Eric Benway

Science Activities

Departing from Woods Hole, we will set a course for BATS 31 40N, 64 10W.  VPR will be deployed upon crossing the shelf break.  XBTs will be deployed hourly.  VPR will be recovered each day at 0930 so CTD station work can begin promptly at 1000.  Upon completion of CTD/pump station work, VPR will be redeployed and underway sampling will recommence.

After sampling at BATS, we will proceed southward along 64W to 20N, then turning southeastward to 10N, 45W.  Alongtrack sampling is flexible, and we will adjust our course according to satellite altimetry and SST / ocean color imagery.

-

Departing from Woods Hole, we will set a course for BATS 31 40N, 64 10W.  VPR will be deployed upon crossing the shelf break.  XBTs will be deployed hourly.  VPR will be recovered each day at 0930 so CTD station work can begin promptly at 1000.  Upon completion of CTD/pump station work, VPR will be redeployed and underway sampling will recommence.

After sampling at BATS, we will proceed southward along 64W to 20N, then turning southeastward to 10N, 45W.  Alongtrack sampling is flexible, and we will adjust our course according to satellite altimetry and SST / ocean color imagery.

on Aug 3, 2010 9:58 AM by Eric Benway

Additional Info

Pre-cruise Planning Meeting: Visit WHOI
- Visit WHOI on Aug 3, 2010 9:58 AM by Eric Benway
Meeting Notes: JULY 14 @ 1400 hrs at 38 Water St. 2nd floor conference room.
- JULY 14 @ 1400 hrs at 38 Water St. 2nd floor conference room. on Aug 3, 2010 9:58 AM by Eric Benway

Stations:

Supporting documentation:

»McGillicuddy_vpr_cruise.PNG
»Tricho_cruise_planning_July_5.pdf
- added McGillicuddy_vpr_cruise.PNG on Sep 29, 2010 2:42 PM by Eric Benway
- McGillicuddy_vpr_cruise_DECK_PLAN.PNG on Sep 29, 2010 2:38 PM by Eric Benway
- added McGillicuddy_vpr_cruise_DECK_PLAN.PNG on Sep 17, 2010 2:52 PM by Eric Benway
- McGillicuddy_vpr_cruise.PNG on Sep 17, 2010 2:51 PM by Eric Benway
- added Tricho_cruise_planning_July_5.pdf on Aug 3, 2010 9:58 AM by Eric Benway
- added McGillicuddy_vpr_cruise.PNG on Sep 17, 2010 2:51 PM by Eric Benway
Notes: LN2 van added onto cruise to support science supply.  Barbados does not have commercially available LN2 any longer.
- -added LN2 van added onto cruise to support science supply.  Barbados does not have commercially available LN2 any longer. on Sep 29, 2010 2:43 PM by Eric Benway

Funding

Funding Agency: NSF
Grant or contract number: OCE-0925284

Scientific Instrumentation for R/V Oceanus


- added VPR winch and block
on Aug 3, 2010 9:58 AM by Eric Benway

Shipboard Equipment

Bathymetry System 12 kHz,
Sippican XBT System (Mark 21),
Bathymetry System 3.5 kHz ,
Deionized Water System,
Fume Hood,
Science Underway Seawater System,
ADCP 150 kHz,
ADCP 75 kHz
-added Bathymetry System 12 kHz on Aug 3, 2010 9:58 AM by Eric Benway
-added Sippican XBT System (Mark 21) on Aug 3, 2010 9:58 AM by Eric Benway
-added Bathymetry System 3.5 kHz on Aug 3, 2010 9:58 AM by Eric Benway
-added ADCP 75 kHz on Aug 3, 2010 9:58 AM by Eric Benway
-added ADCP 150 kHz on Aug 3, 2010 9:58 AM by Eric Benway
-added Science Underway Seawater System on Aug 3, 2010 9:58 AM by Eric Benway
-added Deionized Water System on Aug 3, 2010 9:58 AM by Eric Benway
-added Fume Hood on Aug 3, 2010 9:58 AM by Eric Benway

CTD/Water Sampling

Seapoint STM turbidity sensor,
911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors,
SBE43 oxygen sensor,
Wet Labs ECO-AFL fluorometer,
Wet Labs C*Star transmissometer (660nm wavelength),
Biospherical underwater PAR (1000m depth limit) with reference Surface PAR
-added Seapoint STM turbidity sensor on Aug 3, 2010 9:58 AM by Eric Benway
-added 911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors on Aug 3, 2010 9:58 AM by Eric Benway
-added SBE43 oxygen sensor on Aug 3, 2010 9:58 AM by Eric Benway
-added Wet Labs ECO-AFL fluorometer on Aug 3, 2010 9:58 AM by Eric Benway
-added Wet Labs C*Star transmissometer (660nm wavelength) on Aug 3, 2010 9:58 AM by Eric Benway
-added Biospherical underwater PAR (1000m depth limit) with reference Surface PAR on Aug 3, 2010 9:58 AM by Eric Benway

Critical CTD Sensors

CTD-Related Equipment

ECO/AFL and STM sensor (WHOI owned)
-added ECO/AFL and STM sensor on Aug 3, 2010 9:58 AM by Eric Benway

Hydrographic Analysis Equipment

Salt Bottles (2 cases of 125 ml provided)
-added Salt Bottles (2 cases of 125 ml provided) on Aug 3, 2010 9:58 AM by Eric Benway

MET Sensors

Barometric Pressure,
Wind speed and direction,
Air temperature,
Relative Humidity,
Precipitation,
Short Wave Solar Radiation
-added Barometric Pressure on Aug 3, 2010 9:58 AM by Eric Benway
-added Wind speed and direction on Aug 3, 2010 9:58 AM by Eric Benway
-added Air temperature on Aug 3, 2010 9:58 AM by Eric Benway
-added Relative Humidity on Aug 3, 2010 9:58 AM by Eric Benway
-added Precipitation on Aug 3, 2010 9:58 AM by Eric Benway
-added Short Wave Solar Radiation on Aug 3, 2010 9:58 AM by Eric Benway

Sample Storage

Refrigerator 8.6 cu. ft.,
Freezer -70°C 3.2 cu. ft. ea.,
Chest Freezer (Household type) 0°F
-added Refrigerator 8.6 cu. ft. on Aug 3, 2010 9:58 AM by Eric Benway
-added Chest Freezer (Household type) 0°F on Aug 3, 2010 9:58 AM by Eric Benway
-added Freezer -70°C 3.2 cu. ft. ea. on Aug 3, 2010 9:58 AM by Eric Benway
Storage Notes: Need 2 chest freezers (-20C) in main lab.
- added Need 2 chest freezers (-20C) in main lab. on Aug 3, 2010 9:58 AM by Eric Benway

Navigation

Will you be using Long Base Line (LBL) navigation? no
- added no on Aug 3, 2010 9:58 AM by Eric Benway
How many nets? 0
How many tansponders? 0
Will you be using Doppler/GPS navigation? yes
- added yes on Aug 3, 2010 9:58 AM by Eric Benway

Winches

CTD Winch with .322" Electro-mechanical wire
-added CTD Winch with .322" Electro-mechanical wire on Aug 3, 2010 9:58 AM by Eric Benway
Winch Notes: VPR winch and block
- added VPR winch and block
on Aug 3, 2010 9:58 AM by Eric Benway
Wires Notes: VPR cable: 3 fiber optic, 3 copper 0.322" opto-electric
- added VPR cable: 3 fiber optic, 3 copper 0.322" opto-electric
on Aug 3, 2010 9:58 AM by Eric Benway

Standard Oceanographic Cables

Slip ring required? yes Number of conductors: 3
Non-standard wire required? yes Type: VPR cable
Traction winch required? no Describe: 
Slip Ring revisions

- Slip ring required? : added yes on Aug 3, 2010 9:58 AM by Eric Benway
- Number of Conductors : added 3 on Aug 3, 2010 9:58 AM by Eric Benway
Non-standard wire revisions

- Non-standard wire required? : added yes on Aug 3, 2010 9:58 AM by Eric Benway
- Type : added VPR cable on Aug 3, 2010 9:58 AM by Eric Benway

Specialized Deck Equipment

Mooring Deployment/Recovery Equipment Required: no Type: 
Cruise Specific Science Winch Required: no Type: 
Nets Required: no Type: 

Over the Side Equipment

Will you be bringing any equipment (winches, blocks, etc.) that lowers instruments over the side? yes
Details:
VPR winch and block

Special Requirements

Elecrical Power: yes Identify: Power for VPR
Equipment Handling: no Identify: 
Inter/intraship Communications: no Identify: 
Science Stowage: no Identify: 
Water: no Identify: 
Electrical Power Revisions

- Electrical Power : changed from no to yes on Sep 23, 2010 10:02 AM by Eric Benway
- Electrical Power : added no on Aug 3, 2010 9:58 AM by Eric Benway
- Identify : added Power for VPR on Aug 3, 2010 9:58 AM by Eric Benway

Additional Cruise Items/Activities

Explosive Devices:no
Portable Air Compressors:no
Flammable Gases:yes
Small Boat Operations:no
SCUBA Diving Operations:no

Hazardous Material

Will hazardous material be utilized?yes
- added yes on Aug 3, 2010 9:58 AM by Eric Benway
Describe deployment method and quantity:

Radioactive Material

Radioiosotopes:no

Additional Information

Is night time work anticipated on this cruise?yes
- added yes on Aug 3, 2010 9:58 AM by Eric Benway
Specialized tech support (Seabeam, coring, other):
VPR tech
- added VPR tech on Aug 3, 2010 9:58 AM by Eric Benway
Other required equipment and special needs:
pumping system to be attached to CTD

2 incubators with fold-down legs to be set up on aft deck

6 wire baskets: 3 on 01 deck for carboy storage, 3 on main deck for pump hose.

LN2 delivery in Barbados to recharge dewars for transit back to WHOI.

- added pumping system to be attached to CTD

2 incubators with fold-down legs to be set up on aft deck

6 wire baskets: 3 on 01 deck for carboy storage, 3 on main deck for pump hose.

LN2 delivery in Barbados to recharge dewars for transit back to WHOI.

on Aug 3, 2010 9:58 AM by Eric Benway

Checklist & Notes

Checklist

U.S. Customs Form: yes
- added yes on Aug 3, 2010 10:03 AM by Eric Benway
Diplomatic Clearance: no
- added no on Aug 3, 2010 10:03 AM by Eric Benway
Date Submitted:
Date Approved:
Agent Information:
WOODS HOLE, MA.

Master R/V OCEANUS
Attn: Scientist's Name OC469-1
c/o WHOI
266 Woods Hole Rd.
Woods Hole, MA 02543

Contact: Eric Benway
Phone: (508) 289-3770
Fax: (508) 457-2185
Email: ebenway@whoi.edu
-------------------------------------------------
BRIDGETOWN, BARBADOS:

Master R/V OCEANUS
Attn: Scientist's Name OC469-1
c/o DaCosta Mannings Inc.
Brandons
St. Michael
BARBADOS

Contact:  Glyne St. Hill, Manager
Email: gsthill@dmishipping.com
Phone:(246) 430-4804
Fax: (246) 431-0051

Contact:  David Priddee, Operations Manager
Email Address: dpriddee@dmishipping.com
Tel. no. (246) 430-4819
Cell no. (246) 231-5348

Contact:  Arlin Kellman, Assistant Operations Manager
Email Address: akellman@dmishipping.com
Tel. No. (246) 430-4825
Cell no. (246) 230-4353

Primary Contact:  Anthony Oliver, Operations Coordinator*
Email Address: logistics@dmishipping.com
Tel. no. (246) 430-4881
Cell no. (246) 231-5382
Fax: (246) 431-0878

*Note: All requests and correspondence should be sent to Anthony Oliver at the email address above with copies to David Priddee and Arlin Kellman.


Agent and WHOI contacts should be copied on all communications. It is requested that shipment information of any equipment be communicated to the Agent and to all WHOI contacts


- added WOODS HOLE, MA.

Master R/V OCEANUS
Attn: Scientist's Name OC469-1
c/o WHOI
266 Woods Hole Rd.
Woods Hole, MA 02543

Contact: Eric Benway
Phone: (508) 289-3770
Fax: (508) 457-2185
Email: ebenway@whoi.edu
-------------------------------------------------
BRIDGETOWN, BARBADOS:

Master R/V OCEANUS
Attn: Scientist's Name OC469-1
c/o DaCosta Mannings Inc.
Brandons
St. Michael
BARBADOS

Contact:  Glyne St. Hill, Manager
Email: gsthill@dmishipping.com
Phone:(246) 430-4804
Fax: (246) 431-0051

Contact:  David Priddee, Operations Manager
Email Address: dpriddee@dmishipping.com
Tel. no. (246) 430-4819
Cell no. (246) 231-5348

Contact:  Arlin Kellman, Assistant Operations Manager
Email Address: akellman@dmishipping.com
Tel. No. (246) 430-4825
Cell no. (246) 230-4353

Primary Contact:  Anthony Oliver, Operations Coordinator*
Email Address: logistics@dmishipping.com
Tel. no. (246) 430-4881
Cell no. (246) 231-5382
Fax: (246) 431-0878

*Note: All requests and correspondence should be sent to Anthony Oliver at the email address above with copies to David Priddee and Arlin Kellman.


Agent and WHOI contacts should be copied on all communications. It is requested that shipment information of any equipment be communicated to the Agent and to all WHOI contacts


on Aug 3, 2010 10:03 AM by Eric Benway
Countries:
Notes:
US Customs forms #4455 will need to be filled out with science equipment, such as laptop computers, microscopes, lab equipment with serial numbers.  Anything of significant value and having a serial number will need to be listed. 
Lab supplies, consumables do not need to be listed. 
- added US Customs forms #4455 will need to be filled out with science equipment, such as laptop computers, microscopes, lab equipment with serial numbers.  Anything of significant value and having a serial number will need to be listed. 
Lab supplies, consumables do not need to be listed. 
on Aug 3, 2010 10:03 AM by Eric Benway
Isotope Use Approval: no
- added no on Aug 3, 2010 10:03 AM by Eric Benway
Isotope Notes:
SCUBA Diving: no
- added no on Aug 3, 2010 10:03 AM by Eric Benway

Checklist

SSSG Tech:
Loading at WHOI SEPT 29 and 30th.
- added Loading at WHOI SEPT 29 and 30th.
on Aug 3, 2010 10:03 AM by Eric Benway