Woods Hole Oceanographic Institution

Cruise Planning Synopsis


AT26-10

Ship

R/V Atlantis

Vehicles

ROV Jason
- added ROV Jason on Oct 7, 2013 9:42 AM by Eric Benway

Cruise Party

Stefan Sievert: Chief Scientist, Principal Investigator
Woods Hole Oceanographic Institution Watson Building 207, MS#52 Woods Hole, Ma. USA 02543
+1 508 289 2305
ssievert@whoi.edu
- added Stefan Sievert as Chief Scientist on Oct 7, 2013 9:42 AM by Eric Benway
- added Stefan Sievert as Principal Investigator on Oct 7, 2013 9:42 AM by Eric Benway

Departure: Dec 29, 2013

Puntarenas, Costa Rica

Arrival: Jan 27, 2014

Balboa, Panama

Operations Area

9º50' N EPR, NP13
- changed 9º50' N EPR, NP13 to 9º50' N EPR, NP13 on Oct 9, 2013 1:44 PM by Betsey Doherty
- changed 9º50' N EPR, NP13 to 9º50' N EPR, NP13 on Oct 7, 2013 10:49 AM by Eric Benway
- changed 9º50' N EPR, NP13 to 9º50' N EPR, NP13 on Oct 7, 2013 9:42 AM by Eric Benway
- 9º50' N EPR, NP13 on Oct 7, 2013 9:42 AM by Eric Benway
Lat/Lon: 9° 49.599′ N / 104° 17.599′ W
- set lat to 9° 49.599′ N, set lon to 104° 17.599′ W on Oct 7, 2013 9:42 AM by Eric Benway
Depth Range: 2400 / 2600
- set min to 2400, set max to 2600 on Oct 7, 2013 9:42 AM by Eric Benway
Will the vessel be operating within 200 NM of a foreign country? no

Science Objectives

Deep-sea hydrothermal vents, first discovered in 1977, are ‘poster child’ ecosystems where microbial chemosynthesis rather than photosynthesis is the primary source of organic carbon. Significant gaps remain in our understanding of the underlying microbiology and biogeochemistry of these fascinating ecosystems. Missing are the identification of specific microorganisms mediating critical reactions in various geothermal systems, metabolic pathways used by the microbes, rates of the catalyzed reactions, amounts of organic carbon being produced, and the larger role of these ecosystems in global biogeochemical cycles. To fill these gaps, we are carrying out an interdisciplinary, international hypothesis-driven research program to understand microbial processes and their quantitative importance at deep-sea vents. Specifically, we will address the following objectives:

  1. Determine key relationships between the taxonomic, genetic and functional diversity, as well as the mechanisms of energy and carbon transfer, in deep-sea hydrothermal vent microbial communities.
  2. Identify the predominant metabolic pathways and thus the main energy sources driving chemoautotrophic production in high and low temperature diffuse flow vents.
  3. Determine energy conservation efficiency and rates of aerobic and anaerobic chemosynthetic primary productivity in high and low temperature diffuse flow vents.
  4. Determine gene expression patterns in diffuse-flow vent microbial communities during attachment to substrates and the development of biofilms.
-

Deep-sea hydrothermal vents, first discovered in 1977, are ‘poster child’ ecosystems where microbial chemosynthesis rather than photosynthesis is the primary source of organic carbon. Significant gaps remain in our understanding of the underlying microbiology and biogeochemistry of these fascinating ecosystems. Missing are the identification of specific microorganisms mediating critical reactions in various geothermal systems, metabolic pathways used by the microbes, rates of the catalyzed reactions, amounts of organic carbon being produced, and the larger role of these ecosystems in global biogeochemical cycles. To fill these gaps, we are carrying out an interdisciplinary, international hypothesis-driven research program to understand microbial processes and their quantitative importance at deep-sea vents. Specifically, we will address the following objectives:

  1. Determine key relationships between the taxonomic, genetic and functional diversity, as well as the mechanisms of energy and carbon transfer, in deep-sea hydrothermal vent microbial communities.
  2. Identify the predominant metabolic pathways and thus the main energy sources driving chemoautotrophic production in high and low temperature diffuse flow vents.
  3. Determine energy conservation efficiency and rates of aerobic and anaerobic chemosynthetic primary productivity in high and low temperature diffuse flow vents.
  4. Determine gene expression patterns in diffuse-flow vent microbial communities during attachment to substrates and the development of biofilms.
on Oct 7, 2013 9:42 AM by Eric Benway

Science Activities

The cruise will include 16 dives with DSV Alvin or ROV Jason, to allow time for deployment and collection of experiments and for extensive sampling of discrete vents along the axis of the 9°46’N to 9°53’N segment of the East Pacific Rise (EPR). We propose to deploy experiments/sample a low temperature diffuse flow vent (e.g., Crab Spa; T: 10-30°C), and a higher temperature vent (e.g., L-vent; T: 30-60°C). Furthermore, we will carry out additional opportunistic sampling for a limited set of parameters (e.g., chemistry, 454- pyrotags, FISH, in situ carbon fixation) of vents that fall into these two categories, or that might have developed since the last visit to the site (May 2012). During the course of the cruise we will collect biomass from fluids and biofilms (deployment/recover experimental microbial colonizers) by utilizing a Large Volume Pump (LVP) (McLane WTS 6-1-142LV). We have successfully used this set up in previous cruises and were able to collect up to ~2,000 liters of hydrothermal fluids resulting in enough biomass (cell density is 2-5 x 105 cells per ml) for subsequent ‘omic’ analyses. During large volume filtration, the volume filtered is recorded by the LVP onboard computer and the vent fluid sampled is monitored with a time series temperature probe, H2S, and pH sensors. Fluid samples for chemical analyses will be collected at each deployment site using the isobaric gas-tight samplers, which will also be used to sample fluids from the two focus sites for the shipboard incubations. Finally, we will deployment/recover experimental microbial colonizers to collect microbial biofilms.We have successfully used these devices in previous cruises to collect sufficient biomass for metatranscriptomic analyses.

-

The cruise will include 16 dives with DSV Alvin or ROV Jason, to allow time for deployment and collection of experiments and for extensive sampling of discrete vents along the axis of the 9°46’N to 9°53’N segment of the East Pacific Rise (EPR). We propose to deploy experiments/sample a low temperature diffuse flow vent (e.g., Crab Spa; T: 10-30°C), and a higher temperature vent (e.g., L-vent; T: 30-60°C). Furthermore, we will carry out additional opportunistic sampling for a limited set of parameters (e.g., chemistry, 454- pyrotags, FISH, in situ carbon fixation) of vents that fall into these two categories, or that might have developed since the last visit to the site (May 2012). During the course of the cruise we will collect biomass from fluids and biofilms (deployment/recover experimental microbial colonizers) by utilizing a Large Volume Pump (LVP) (McLane WTS 6-1-142LV). We have successfully used this set up in previous cruises and were able to collect up to ~2,000 liters of hydrothermal fluids resulting in enough biomass (cell density is 2-5 x 105 cells per ml) for subsequent ‘omic’ analyses. During large volume filtration, the volume filtered is recorded by the LVP onboard computer and the vent fluid sampled is monitored with a time series temperature probe, H2S, and pH sensors. Fluid samples for chemical analyses will be collected at each deployment site using the isobaric gas-tight samplers, which will also be used to sample fluids from the two focus sites for the shipboard incubations. Finally, we will deployment/recover experimental microbial colonizers to collect microbial biofilms.We have successfully used these devices in previous cruises to collect sufficient biomass for metatranscriptomic analyses.

on Oct 7, 2013 9:42 AM by Eric Benway

Additional Info

Pre-cruise Planning Meeting: Visit WHOI
- Visit WHOI on Oct 7, 2013 9:42 AM by Eric Benway
- on Oct 7, 2013 9:42 AM by Eric Benway
Media personnel on board: Video, Writer
- Video, Writer on Oct 7, 2013 9:42 AM by Eric Benway
This cruise will be featured on Dive & Discover
- This cruise will be featured on Dive & Discover on Oct 7, 2013 9:42 AM by Eric Benway

Stations:

  Station 1
  Distance: 1,150
  Days: 16
  Latitude: 9° 49.599’ N
  Longitude: 104° 17.599’ E
- added Name: Lat: 9° 49.599’ N, Lon: 104° 17.599’ E, Distance: 1,150, Days: 16 on Oct 7, 2013 9:42 AM by Eric Benway

Supporting documentation:

- -added on Oct 7, 2013 9:42 AM by Eric Benway

Funding

Funding Agency: NSF
Grant or contract number: 1136727

Scientific Instrumentation for R/V Atlantis

Shipboard Equipment

Deionized Water System,
Fume Hood,
Multibeam ,
Navigation - Heading,
Relay Transponder for Wire Use,
Transponder Navigation - Sonardyne USBL,
Science Underway Seawater System
-added Deionized Water System on Oct 7, 2013 9:42 AM by Eric Benway
-added Fume Hood on Oct 7, 2013 9:42 AM by Eric Benway
-added Multibeam on Oct 7, 2013 9:42 AM by Eric Benway
-added Navigation - Heading on Oct 7, 2013 9:42 AM by Eric Benway
-added Relay Transponder for Wire Use on Oct 7, 2013 9:42 AM by Eric Benway
-added Transponder Navigation - Sonardyne USBL on Oct 7, 2013 9:42 AM by Eric Benway
-added Science Underway Seawater System on Oct 7, 2013 9:42 AM by Eric Benway

Shipboard Communication

Basic Internet access via HiSeasNet,
Is there a need to receive data from shore on a regular basis?,
Is there a need to transfer data to shore on a regular basis?,
Is there an expectation to use Skype or any other real-time video conference program?
-added Basic Internet access via HiSeasNet on Oct 7, 2013 9:42 AM by Eric Benway
-added Is there a need to receive data from shore on a regular basis? on Oct 7, 2013 9:42 AM by Eric Benway
-added Is there a need to transfer data to shore on a regular basis? on Oct 7, 2013 9:42 AM by Eric Benway
-added Is there an expectation to use Skype or any other real-time video conference program? on Oct 7, 2013 9:42 AM by Eric Benway

CTD/Water Sampling

911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors,
SBE43 oxygen sensor,
Wet Labs FLNTURTD Combination Flourometer and Turbidity Sensor
-added 911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors on Oct 7, 2013 9:42 AM by Eric Benway
-added SBE43 oxygen sensor on Oct 7, 2013 9:42 AM by Eric Benway
-added Wet Labs FLNTURTD Combination Flourometer and Turbidity Sensor on Oct 7, 2013 9:42 AM by Eric Benway

Critical CTD Sensors

-removed Dissolved Oxygen Titration System (Brinkmann Titrator) on Dec 2, 2013 10:58 AM by Eric Benway
-removed Oxygen Sample Bottles (available in 150 ml sizes) on Dec 2, 2013 10:58 AM by Eric Benway
-added Dissolved Oxygen Titration System (Brinkmann Titrator) on Oct 7, 2013 9:42 AM by Eric Benway
-added Oxygen Sample Bottles (available in 150 ml sizes) on Oct 7, 2013 9:42 AM by Eric Benway

Sample Storage

Climate Controlled Walk-in,
Freezer -70°C 25 cu. ft.,
Freezer -70°C 3.2 cu. ft. ea.,
Refrigerator 8.6 cu. ft.,
Scientific Walk-in Freezer
-added Climate Controlled Walk-in on Oct 7, 2013 9:42 AM by Eric Benway
-added Freezer -70°C 25 cu. ft. on Oct 7, 2013 9:42 AM by Eric Benway
-added Freezer -70°C 3.2 cu. ft. ea. on Oct 7, 2013 9:42 AM by Eric Benway
-added Refrigerator 8.6 cu. ft. on Oct 7, 2013 9:42 AM by Eric Benway
-added Scientific Walk-in Freezer on Oct 7, 2013 9:42 AM by Eric Benway
Storage Notes: Will need to keep samples on board until first USA port call early Feb 2014.   Geoff Wheat cruise will occupy freezer space as well.
- added Will need to keep samples on board until first USA port call early Feb 2014.   Geoff Wheat cruise will occupy freezer space as well. on Oct 7, 2013 9:44 AM by Eric Benway

Navigation

Will you be using Long Base Line (LBL) navigation? no
- added no on Oct 7, 2013 9:42 AM by Eric Benway
How many nets? null
How many tansponders? null
Will you be using Ultra-short baseline (USBL) navigation?no
- added no on Oct 7, 2013 9:42 AM by Eric Benway
Navigation Notes: not sure what's required for Jason
- added not sure what's required for Jason on Oct 7, 2013 9:42 AM by Eric Benway

Winches

CTD Winch with .322" Electro-mechanical wire,
Hydro Winch with .25" hydro wire
-added CTD Winch with .322" Electro-mechanical wire on Oct 7, 2013 9:42 AM by Eric Benway
-added Hydro Winch with .25" hydro wire on Oct 7, 2013 9:42 AM by Eric Benway
Winch Notes:

Wire use and application

CTD Winch with .322" Electro-mechanical wire,
Hydro Winch with .25" hydro wire
-added CTD Winch with .322" Electro-mechanical wire on Oct 7, 2013 9:42 AM by Eric Benway
-added Hydro Winch with .25" hydro wire on Oct 7, 2013 9:42 AM by Eric Benway
Winch Notes:

Standard Oceanographic Cables

Slip ring required? no
Non-standard wire required? no
Traction winch required? no

Portable Vans

Chemical Storage Van
-added Chemical Storage Van on Oct 7, 2013 9:42 AM by Eric Benway

Specialized Deck Equipment

Mooring Deployment/Recovery Equipment Required: no Type: 
Cruise Specific Science Winch Required: no Type: 
Nets Required: no Type: 

Over the Side Equipment

Will you be bringing any equipment (winches, blocks, etc.) that lowers instruments over the side? yes
Details: A McLane in situ large volume pump is going to be deployed as an elevator. It is planned to bring the pump close to the deplyment site the night before a dive by wire and then to position at the site with Jason the next day. Either on the same dive or the following dive Jason is going to release the pump. This procedure has been tested very succesfully on previous cruises, the latest being AT15-38. For the deplyment of the pump we will need weights (5 plates) and floats (4 spheres). We plan for ~6 deployments
- changed from A McLane in situ large volume pump is going to be deployed as an elevator. It is planned to bring the pump close to the deplyment site the night before a dive by wire and then to position at the site with Alvin the next day. Either on the same dive or the following dive Alvin is going to release the pump. This procedure has been tested very succesfully on previous cruises, the latest being AT15-38. For the deplyment of the pump we will need weights (5 plates) and floats (4 spheres). We plan for ~6 deployments to A McLane in situ large volume pump is going to be deployed as an elevator. It is planned to bring the pump close to the deplyment site the night before a dive by wire and then to position at the site with Jason the next day. Either on the same dive or the following dive Jason is going to release the pump. This procedure has been tested very succesfully on previous cruises, the latest being AT15-38. For the deplyment of the pump we will need weights (5 plates) and floats (4 spheres). We plan for ~6 deployments on Oct 7, 2013 9:44 AM by Eric Benway
- added A McLane in situ large volume pump is going to be deployed as an elevator. It is planned to bring the pump close to the deplyment site the night before a dive by wire and then to position at the site with Alvin the next day. Either on the same dive or the following dive Alvin is going to release the pump. This procedure has been tested very succesfully on previous cruises, the latest being AT15-38. For the deplyment of the pump we will need weights (5 plates) and floats (4 spheres). We plan for ~6 deployments on Oct 7, 2013 9:42 AM by Eric Benway

Special Requirements

Elecrical Power: no Identify 
Equipment Handling: no Identify: 
Inter/intraship Communications: no Identify: 
Science Stowage: no Identify: 
Water: no Identify: 

Additional Cruise Items/Activities

Explosive Devices:no
Portable Air Compressors:no
Flammable Gases:yes
Small Boat Operations:no
SCUBA Diving Operations:no

Hazardous Material

Will hazardous material be utilized?yes
- added yes on Oct 7, 2013 9:42 AM by Eric Benway
Describe deployment method and quantity:

Radioactive Material

Radioiosotopes:no

Additional Information

Is night time work anticipated on this cruise?yes
- added yes on Oct 7, 2013 9:42 AM by Eric Benway
Specialized tech support (Seabeam, coring, other):
Other required equipment and special needs:
Dec 2, 2013 _ Eric B. took off O2Titrator.  Stefan confirmed they will not require this.
- added Dec 2, 2013 _ Eric B. took off O2Titrator.  Stefan confirmed they will not require this. on Dec 2, 2013 10:58 AM by Eric Benway

<Could not load the preview of ROV Jason Information data >

Checklist & Notes

Checklist

U.S. Customs Form: no
- added no on Oct 7, 2013 9:50 AM by Eric Benway
Diplomatic Clearance: no
- added no on Oct 7, 2013 9:50 AM by Eric Benway
Date Submitted:
Date Approved:
Agent Information:
San Diego pre mobilzation Nov 15-16.

Master R/V Atlantis
Attn: Scientist's Name - AT26-10
c/o Paxton, Shreve & Hays Inc.
453 54th Street Suite 101
San Diego, CA   92114

Contact: Tom Jenkins
Phone: (619) 232-8941
Fax: (619) 232-3006
Telex: 6731029 SHREVE SDG
Email: marineops@pshinc.net

SHIP WILL BE DOCKED at 10th Avenue Marine Terminal
623 Switzer St. San Diego CA. 92101

COSTA RICA
Master R/V Atlantis
Attn: Scientist's Name - AT26-10
IN TRANSIT
c/o Vasile Tudoran Transport
819 Ohio Ave.
Long Beach, CA 90804

Contact: Vasile Tudoran
Phone: (562) 882-5590
Fax: (562) 434-9800
Email: vtudoran@aol.com

Local Address for Large Shipments:

Master R/V Atlantis
Attn: Scientist's Name - AT26-10
IN TRANSIT
SERMAR SA
Aptdo 76
Puntarenas, Costa Rica
Attn:
Manuel Fernandez
Phone: 011-506-2661-1529
Fax: 011-506-2661-2779

Manuel Fernandez, cell #011 506 8862 6787

Note: Agent and WHOI contacts should be copied on all communications. It is requested that shipment information of any equipment be communicated to the Agent and WHOI contacts
- added San Diego pre mobilzation Nov 15-16.

Master R/V Atlantis
Attn: Scientist's Name - AT26-10
c/o Paxton, Shreve & Hays Inc.
453 54th Street Suite 101
San Diego, CA   92114

Contact: Tom Jenkins
Phone: (619) 232-8941
Fax: (619) 232-3006
Telex: 6731029 SHREVE SDG
Email: marineops@pshinc.net

SHIP WILL BE DOCKED at 10th Avenue Marine Terminal
623 Switzer St. San Diego CA. 92101

COSTA RICA
Master R/V Atlantis
Attn: Scientist's Name - AT26-10
IN TRANSIT
c/o Vasile Tudoran Transport
819 Ohio Ave.
Long Beach, CA 90804

Contact: Vasile Tudoran
Phone: (562) 882-5590
Fax: (562) 434-9800
Email: vtudoran@aol.com

Local Address for Large Shipments:

Master R/V Atlantis
Attn: Scientist's Name - AT26-10
IN TRANSIT
SERMAR SA
Aptdo 76
Puntarenas, Costa Rica
Attn:
Manuel Fernandez
Phone: 011-506-2661-1529
Fax: 011-506-2661-2779

Manuel Fernandez, cell #011 506 8862 6787

Note: Agent and WHOI contacts should be copied on all communications. It is requested that shipment information of any equipment be communicated to the Agent and WHOI contacts
on Oct 7, 2013 9:50 AM by Eric Benway
Countries:
Embarkation port Puntarenas Costa Rica
Disembark port will be Balboa Panama.
- added Embarkation port Puntarenas Costa Rica
Disembark port will be Balboa Panama.
on Oct 7, 2013 9:50 AM by Eric Benway
Notes:
US 4455's custom declaration forms to be submitted to Eric Benway by Nov 21 2013.
- changed from US 4455's custom declaration forms to be submitted to Eric Benway by Nov 21 2013. to US 4455's custom declaration forms to be submitted to Eric Benway by Nov 14 2013. on Oct 7, 2013 11:11 AM by Eric Benway
- changed from US 4455's custom declaration forms to be submitted to Eric Benway by Nov 14 2013. to US 4455's custom declaration forms to be submitted to Eric Benway by Nov 21 2013. on Nov 7, 2013 10:53 AM by Eric Benway
- added US 4455's custom declaration forms to be submitted to Eric Benway by Nov 14 2013. on Oct 7, 2013 9:50 AM by Eric Benway
Isotope Use Approval: no
- added no on Oct 7, 2013 9:50 AM by Eric Benway
Isotope Notes:
no rads on this cruise.
- removed answer no rads on this cruise. on Oct 7, 2013 11:11 AM by Eric Benway
- added no rads on this cruise. on Nov 7, 2013 10:53 AM by Eric Benway
SCUBA Diving: no
- added no on Oct 7, 2013 9:50 AM by Eric Benway

Checklist

SSSG Tech:
Allison Heater, Catie Graver
- added Allison Heater, Catie Graver on Oct 7, 2013 9:50 AM by Eric Benway
Pre load equipment, chems and gases in San Dieog CA. Nov 22, 23.  All customs documents to Eric By Nov 21.   Items to be loaded into the science hold. 

Mob will take place Dec 26 - Dec 28 in Puntarenas CR.  Ship will be at anchor and launches will be schedued from shore to ship.

Demob in Balboa Panama Jan 28, 29
- changed from Pre load equipment, chems and gases in San Dieog CA. Nov 22, 23.  All customs documents to Eric By Nov 21.   Items to be loaded into the science hold. 

Mob will take place Dec 26 - Dec 28 in Puntarenas CR.  Ship will be at anchor and launches will be schedued from shore to ship.

Demob in Balboa Panama Jan 28, 29
to Pre load equipment, chems and gases in San Dieog CA. Nov 15-16.  All customs documents to Eric By Nov 14.   Items to be loaded into the science hold. 

Mob will take place Dec 26 - Dec 28 in Puntarenas CR.  Ship will be at anchor and launches will be schedued from shore to ship.

Demob in Balboa Panama.
on Oct 7, 2013 11:11 AM by Eric Benway
- changed from Pre load equipment, chems and gases in San Dieog CA. Nov 15-16.  All customs documents to Eric By Nov 14.   Items to be loaded into the science hold. 

Mob will take place Dec 26 - Dec 28 in Puntarenas CR.  Ship will be at anchor and launches will be schedued from shore to ship.

Demob in Balboa Panama.  
to Pre load equipment, chems and gases in San Dieog CA. Nov 22, 23.  All customs documents to Eric By Nov 21.   Items to be loaded into the science hold. 

Mob will take place Dec 26 - Dec 28 in Puntarenas CR.  Ship will be at anchor and launches will be schedued from shore to ship.

Demob in Balboa Panama Jan 28, 29
on Nov 7, 2013 10:53 AM by Eric Benway
- added Pre load equipment, chems and gases in San Dieog CA. Nov 15-16.  All customs documents to Eric By Nov 14.   Items to be loaded into the science hold. 

Mob will take place Dec 26 - Dec 28 in Puntarenas CR.  Ship will be at anchor and launches will be schedued from shore to ship.

Demob in Balboa Panama.  
on Oct 7, 2013 9:50 AM by Eric Benway