Woods Hole Oceanographic Institution

Cruise Planning Questionnaire

Pickled Protists2


R/V Atlantis


ROV Jason

Cruise Party

Joan Bernhard: Principal Investigator
Woods Hole Oceanographic Institution Watson Building 219, MS#52 Woods Hole, Ma. USA 02543
+1 508 289 3480

Virginia Edgcomb: Chief Scientist, Principal Investigator
Woods Hole Oceanographic Institution Watson Building 213, MS#52 Woods Hole, Ma. USA 02543
+1 508 289 3734

Departure: Piraeus, Greece on Nov 21, 2011

Arrival: Piraeus, Greece on Dec 5, 2011

Mobilization Date: Nov 19, 2011

Demobilization Date: Dec 6, 2011

Supporting documentation:

Operations Area: E. Mediterranean

Lat/Lon: 35° 19.213′ N / 21° 41.351′ E

Depth Range: 2000 / 6000

Will the vessel be operating within 200 NM of a foreign country? Greece
Are visas or special travel documents required? no

Science objectives

1. Water column sampling at two basins: Discovery and Urania Basins, at 3 depths: brine (approx 3500-4000m depth), halocline (~3500m), and reference (~2000m) using a new sampler, the SID-ISMS (under construction). Niskin rosette as backup.
2. Sediment coring at both basins, using ROV Jason. Cores will be collected in 3 locations for each basin, the "bathtub ring" where the halocline impinges on the seafloor, the brine, and a reference core sample from above the halocline.

Science Activities

1. Water column sampling using the SID-ISMS to collect in situ filtered water (ship must hold position during deployment while instrument is working) and preserved in situ for molecular work.
2. Water column sampling using the SID-ISMS to collect in situ filtered and preserved samples for FISH/microscopy experiments.
3. Grazing experiment using SID-ISMS to collect water from halocline of each basin and measure the grazing rates of protozoa over a 6 hour period. Instrument must stay down at depth during the 6 hours. Sampler can be lifted to ~3000 m depth to get it away from the bottom., but ship must stay in position to avoid dragging the sampler
4. Coring of "bathtub ring" at each basin using the ROV Jason which will be used to locate the bathtub ring and then collect cores at that location.
5. Coring of brine at each basin. ROV Jason will reach into the brine from the bathtub ring area and will collect cores.  Corers will be a combination of large Jason pushcores (Bernhard owns a number of these and will bring them) and also some "Tim Shank type RNAlater samplers".  These need to be fabricated (and perhaps some borrowed from Shank). 
6. Coring of a reference sample from outside the halocline (above) at each basin (normal seawater sediments).

Pre-cruise planning meeting: Visit WHOI

we have date for Thursday at 3pm
Media personnel on board: Video, Writer
Dive and Discover


  Station 1
  Distance: 20
  Days: 6
  Latitude: 35° 13.674’ N
  Longitude: 21° 28.58’ E

  Distance: 200
  Days: 6
  Latitude: 35° 19.213’ N
  Longitude: 21° 41.351’ E

Funding Agency: NSF Bio Oce #OCE 1061391, NSF #84957800/OCE-0849578

- added NSF Bio Oce #OCE 1061391 on May 5, 2011 11:39 AM by Joan M. Bernhard
- added NSF #84957800/OCE-0849578 on May 3, 2011 3:06 PM by Virginia P. Edgcomb

R/V Atlantis

Shipboard Equipment

ADCP 150 kHz
Bathymetry System 12 kHz
Bathymetry System 3.5 kHz
Deionized Water System
Fume Hood
Navigation - Heading
Navigation - Position
Transponder Navigation - Sonardyne USBL
Science Underway Seawater System

Shipboard Communication

Basic Internet access via HiSeasNet
Is there a need to receive data from shore on a regular basis?
Is there a need to transfer data to shore on a regular basis?

CTD/Water Sampling

911+ Rosette 24-position, 10-liter bottle Rosette with dual T/C sensors
SBE43 oxygen sensor
Wet Labs C*Star transmissometer (660nm wavelength)

Critical CTD Sensors: 

Hydrographic Analysis Equipment


MET Sensors

Air temperature
Barometric Pressure
Relative Humidity
Wind speed and direction

Sediment Sampling


Sample Storage

Climate Controlled Walk-in
Freezer -70°C 25 cu. ft.
Refrigerator 8.6 cu. ft.
Scientific Walk-in Freezer

Storage Notes:


Will you be using Long Base Line (LBL) navigation? no

Will you be using Ultra-short baseline (USBL) navigation for other than Alvin operations? no



Navigation Notes: I am not up on exactly what Jason needs, but I know it used to be LBL, but seem to recall Jason uses USBL now?  We may need to set out transponders. 


CTD Winch with .322" Electro-mechanical wire
Trawl Winch with 9/16th trawl wire

Winch Notes:

Wire use and application

CTD Winch with .322" Electro-mechanical wire

Wire Notes: This wire (the regular CTD wire) will be used for the SID/ISMS, which has real-time communication features. The instrument is capable of carrying up to 8L of water. The rest of the instrument is filter units. Edgcomb can not provide an exact weight at this time since it is under construction, but it is far lighter than a Niskin rosette
Slip ring required? no Number of conductors: 
Non-standard wire required? no Type: 
Traction winch required? no Describe: 

Portable Vans

Chemical Storage Van

Other Science Vans:

Specialized Deck Equipment

Mooring Deployment/Recovery Equipment Required: no Type: 
Cruise Specific Science Winch Required: no Type: 
Nets Required: no Type: 

Over the Side Equipment

Will you be bringing any equipment (winches, blocks, etc.) that lowers instruments over the side? no

Special Requirements

Elecrical Power: yes Identify: nothing out of the ordinary
Equipment Handling: no Identify: 
Inter/intraship Communications: no Identify: 
Science Stowage: no Identify: 
Water: yes Identify: DI water

Additional Cruise Items/Activities

Explosive Devices: no
Portable Air Compressors: no
Flammable Gases: no
Small Boat Operations: no
SCUBA Diving Operations: no

Hazardous Material

Will hazardous material be utilized? yes

Describe deployment method and quantity:
will provide a complete list but for now:

Bouin's fixative
Lugol's fixative

Radioactive Material

Radioiosotopes: no

Additional Information

Is night time work anticipated on this cruise? yes

Specialized tech support (Seabeam, coring, other): We will deploy the SID/ISMS during night time for the grazing experiments which run min. 6 hrs. This will occur on 4 nights (two each basin)

Other required equipment and special needs: 

ROV Jason

Site Survey

Will you provide detailed charts of the work area(s)?  no

Current Chart(s):

If no, willl you need Jason to generate maps of the work area(s)?  no

Will you need post-dive maps of the work area generated?  no


Will you be using Long Base Line (LBL) navigation? no

Will you be using Ultra-short baseline (USBL) navigation? yes

Will you be using Doppler/GPS navigation? yes

What type of samples do you expect to collect?


Will you be using elevators to transport samples to the surface?  no

If yes, how many would you anticipate? 

Science Supplied Equipment

Are you supplying equipment to be used on HOV Jason?

Has this equipment been used on Jason before?  no

Please give a brief description of the equipment, its intended purpose, the cruise # it was last used on if any and its deployment method.

Does this equipment use an external pressure housing?  no

If yes, what is the pressure rating? 
and test pressure? 

Or has the pressure case been tested per Alvin Pressure Test requirements?  no

Housing schematic with dimensions and include air and water weights.

Does the equipment have an associated computer or control panel for remote operation from the personnel sphere?  no

Air weight of this equipment? 

Does the equipment require data or a power interface from the vehicle?  no

Does this equipment require hydraulic inputs from the vehicle?  no
Hydraulic schematic of the equipment requirements.

Does this equipment require manipulation?  no

If yes, please describe how the equipment is to be manipulated.

Will this equipment be deployed off the vehicle?  no

If yes, please describe how the equipment is intended for deployment.

If yes, will the equipment be disconnected from the vehicle and left in situ?  no

How long will the deployment be? 

Will the equipment be recovered by the same vehicle?  no

If recovering equipment deployed with another vehicle, provide pressure rating: 
and test pressure: 

Does this equipment use any glass spheres for either buoyancy or as pressure housings?  no

Hazardous Material

Will hazardous material be utilized? no

Additional Information

Brief operations description or comments:

Date Submitted: Sep 6, 2011 8:46 PM by Eric Benway